Analysis of circulating breast cancer cell heterogeneity and interactions with peripheral blood mononuclear cells.

ABSTRACT: For solid tumors, extravasation of cancer cells and their survival in circulation represents a critical stage of the metastatic process that lacks complete understanding. Gaining insight into interactions between circulating tumor cells (CTCs) and other peripheral blood mononuclear cells (PBMCs) may provide valuable prognostic information. The purpose of this study was to use single-cell RNA-sequencing (scRNA-seq) of liquid biopsies from breast cancer patients to begin defining intravascular interactions. We captured CTCs from the peripheral blood of breast cancer patients using size-exclusion membranes followed by scRNA-seq of enriched CTCs and carry-over PBMCs. Transcriptome analysis identified two populations of CTCs: one enriched for transcripts indicative of estrogen responsiveness an

SUBMITTER: Brechbuhl HM 

PROVIDER: S-EPMC7895311 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature