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ABSTRACT: Bacterial Attachment to Microplastics

PROVIDER: PRJNA587708 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10396400_1.fastq.gz	Fastqsanger.gz
	SRR10396400_2.fastq.gz	Fastqsanger.gz
	SRR10396401_1.fastq.gz	Fastqsanger.gz
	SRR10396401_2.fastq.gz	Fastqsanger.gz
	SRR10396402_1.fastq.gz	Fastqsanger.gz

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Exposure to microplastics reduces attachment strength and alters the haemolymph proteome of blue mussels (Mytilus edulis)

Project description:The contamination of marine ecosystems with microplastics, such as the polymer polyethylene, a commonly used component of single-use packaging, is of global concern. Although it has been suggested that biodegradable polymers, such as polylactic acid, may be used to replace some polyethylene packaging, little is known about their effects on marine organisms. Blue mussels, Mytilus edulis, have become a “model organism” for investigating the effects of microplastics in marine ecosystems. We show here that repeated exposure, over a period of 52 days in an outdoor mesocosm setting, of M. edulis to polyethylene microplastics reduced the number of byssal threads produced and the attachment strength (tenacity) by ~50%. Exposure to either type of microplastic altered the haemolymph proteome and, although a conserved response to microplastic exposure was observed, overall polyethylene resulted in more changes to protein abundances than polylactic acid. Many of the proteins affected are involved in vital biological processes, such as immune- and stress- regulation, metabolism and cellular and structural development. Our study highlights the utility of mass spectrometry-based proteomics to assess the health of key marine organisms and identifies the potential mechanisms by which microplastics, both conventional and biodegradable, could affect their ability to form and maintain reefs.

2019-01-02 | PXD011567 | Pride

Polystyrene nano- and microplastic particles induce an inflammatory gene expression profile in neural stem cell-derived astrocytes

Project description:Cellular uptake and cytotoxicity data from neural cells treated with microplastics were compared and contrasted. Transcriptomic data obtained by RNA-seq from astrocytes treated with microplastics was assessed further.

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Pollutants bioavailability and toxicological risk from microplastics to marine mussels

Project description:Microplastics represent a growing environmental concern for the oceans due to their potential capability to adsorb different classes of pollutants, thus representing a still unexplored source of exposure for aquatic organisms. In this study polystyrene (PS) microplastics were characterized for their capability to adsorb pyrene (PYR) as model compound for polycyclic aromatic hydrocarbons, and transfer this chemical to filter feeding mussels Mytilus galloprovincialis. Gene expression analyses of Mytilus galloprovincialis exposed to polystyrene (PS) microplastics and to polystyrene contaminated with pyrene (PS-PYR) have been performed trough a DNA microarray platform.

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Project description:Bacterial assemblage on microplastics

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Polyethylene terephthalate microplastics generated from disposable water bottles induce interferon signalling pathway in mouse lung epithelial cells

Project description:Polyethylene terephthalate microplastics generated from disposable water bottles induce interferon signalling pathway in mouse lung epithelial cells

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RNA-Seq analysis of wild type Pseudomonas aeruginosa adhered to a surface for 5, 10, 15, 30, 45, or 60min

Project description:Purpose: Use RNA-seq to investigate the kinetics of bacterial response during the first hour of surface attachment Methods: Logarithmic phase bacterial culture in LB with optical density 600 (OD600) of 0.5 was inoculated in an Ibidi μ-Slide. At the indicated time from 5 - 60 min post-attachment, the channel was rinsed twice in PBS to remove non-adherent cells and 200μL of Trizol was added to the channel. Trizol was collected and stored at -80°C for RNA isolation. HiSeq 4000 sequencing was performed, generating approximately 300 million total paired end 300 bp reads from the 15 total samples, with a mean quality score of 37.4. Reads were aligned to the reference PAO1 genome using Rockhopper. Results:In total, 453 genes were differentially regulated between 5 min post-attachment, and at least one of the later time points. Expression of most of the regulated genes was elevated (390 genes) rather than reduced (63 genes) upon surface attachment Conclusion: This rapid response indicates that biofilm phenotypes may occur more rapidily than previously proposed, and that the phenotype may be triggered by surface contact

2022-01-28 | GSE194320 | GEO

Bacterial communities on microplastics

Project description:Bacterial communities on microplastics Raw sequence reads

| PRJNA564193 | ENA

Project description:Bacterial infomation under the influence of microplastics

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Project description:Microplastics inhibited the nodular endophytic bacterial community

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Project description:bacterial composition of biofilm on artificial microplastics

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