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Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells

ABSTRACT: Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells

PROVIDER: PRJNA588396 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10421200.fastq.gz	Fastqsanger.gz
	SRR10421300.fastq.gz	Fastqsanger.gz
	SRR10421400.fastq.gz	Fastqsanger.gz
	SRR10421500.fastq.gz	Fastqsanger.gz
	SRR10421600.fastq.gz	Fastqsanger.gz

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Single-cell RNA sequencing on EM-E6/E7/TERT-1 endometrial epithelial cells

Project description:We report the single-cell high-throughput sequencing for non-tumorigenic EM-E6/E7/TERT-1(EME6/7t) endometrial epithelial cell line. EME6/7t cells were exposed to condition medium (CM) of adipose-derived stromal cells (ASCs) with or without tiplaxtinin (PAI-039). We employed unsupervised clustering analysis to characterize various PAI-1 related regulatory models in EME6/7t cells. This study provides a framework for exploring heterogeneous gene expression patterns caused by plasminogen activator inhibitor-1 (PAI-1) responses in the tumor microenvironment.

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Project description:The infection with high-risk human papillomavirus is aetiologically linked to cervical cancer, the role of miRNAs regulated by virus oncogene in cancer progression remain largely unknown. Here, we screened the differentially expressed miRNAs with miRNA array between virus oncogene e6/e7 silenced and not in HPV16-positive cervical cancer cell lines In the study, we screened the differentially expressed miRNAs with miRNA array (Exiqon, miRCURY LNA microRNA array, 7th gen [hsa, miRBase 18]) between virus oncogene e6/e7 silenced and not in HPV16-positive cervical cancer cell lines to found miRNAs regulated by virus oncogene e6/e7. Biological replicates: 3 control, 3 e6/e7 silenced, independently grown and harvested. four replicates per array.

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