Project description:Dental mesenchymal cells (dMCs) differentiate into dentin-secreting odontoblasts and are utilized in the majority of tooth regeneration studies to produce an entire tooth organ. An in vitro culture platform of dMCs is useful in generating an adequate quantity of cells for experiments and regenerative therapy. However, culturing techniques may induce functional modification in dMCs. Here, we examined the transcriptomic alterations induced by in vitro culture and investigated the biological properties of cultured dMCs. Hierarchical clustering analysis and principal components analysis reveal an overall transcriptional similarity between the first and second passages that differed significantly from freshly obtained cells. Following in vitro culture, the biological processes of cell adhesion, chromatin organization, cell differentiation and development are significantly altered. Components of signaling pathways, including p53, transforming group factor β/bone morphogenetic protein (TGFβ/BMP), Hedgehog, and Wnt, as well epigenetic regulators are involved. Furthermore, the osteogenic potential of cultured dMCs is also impaired. Our study provides valuable insights for the modification of the current culturing methods to maintain the characteristics of in vivo dMCs.

2017-02-09 | GSE65164 | GEO

Comparative transcriptome of cancer cells cultured in MGS

Project description:Comparative transcriptome of cancer cells cultured in MGS

| PRJNA1011219 | ENA

Alteration of gene expressions in RCC cell lines co-cultured with HGECs with down-regulated INSR

Project description:RCC cell lines (786-O cell line and Caki-1 cell line)were co-cultured with human renal glomerular endothelial cells (HGECs) treated with si-RNA of INSR (HGEC-siINSR). Gene expression alteration was analyzed using microarray.

2023-04-27 | GSE230698 | GEO

Transcriptome analysis of cultured human alveolar epithelial type 2 cells

Project description:Transcriptome analysis of cultured human alveolar epithelial type 2 cells

| PRJNA527026 | ENA

Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

Project description:Temperature dependence of the transcriptome in cultured Drosophila S2R+ cells

| PRJNA668006 | ENA

Single cell transcriptome of cultured thymic progenitor and stem cells

Project description:Single cell transcriptome of cultured thymic progenitor and stem cells

| PRJNA909231 | ENA

Comparative transcriptome of cancer cells cultured in a conventional manner

Project description:Comparative transcriptome of cancer cells cultured in a conventional manner