Project description:4T1 mammary cancer cells exihibit higher proliferation and metastatic ability when cultured in conditioned medium from mesenchymal stem cells . Also, co-implantation with MSCs, 4T1 tumors show higher tumor growth and metastasis. We used microarrays to detail the global programme of gene expression underlying the alteration of cell behaviour RNA was extrated from 4T1 cells cultured in MSC-CM or regular medium as well as 4T1 cells isolated from co-cultured with MSCs. Gene expression of these cell groups was acquired from Affymetrix microarray analysis using mouse 430 2.0 array chip.

2012-06-06 | E-GEOD-38251 | biostudies-arrayexpress

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

Project description:Transcriptional profiling of cancer stem cells (sphere-cultured cells) comparing non-cancer stem cells (adherent-cultured cells). Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 sphere-cultured replicate and 1 adherent-culture replicate.

2016-04-01 | E-GEOD-77150 | biostudies-arrayexpress

Comparative transcriptome of cancer cells cultured in MGS

Project description:Comparative transcriptome of cancer cells cultured in MGS

| PRJNA1011219 | ENA

Gene expression of canine mammary cancer stem-like cells co-cultured with tumor associated macrophages

Project description:The aim of our study was to evaluate gene expression of canine mammary cancer stem-like cells co-cultured with tumor associated macrophages. Two canine mammary cancer cell lines (CMT-U27 and P114) were stained using anti-Sca1 (stem cell antigen 1), anti-EpCAM (Epithelial cell adhesion molecule) and anti-CD44 antibody. The FACS analysis showed 0,02-0,05% of Sca1+/EpCAM+/CD44+ in each of the cell line. Cancer stem-like cells were collected using FACS Aria II then co-cultured with tumor associated macrophages and used for further analysis of gene expression ( using Agilent Gene Expression Hybridization Kit ).

2016-06-01 | GSE73230 | GEO

Transcriptomes of cultured human hematopoietic stem cells.

Project description:Transcriptomes of cultured human hematopoietic stem cells.

| PRJNA791215 | ENA