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Transcriptional profiling of ependymal cells lining the ventricles in the central nervous system of a Dcdc2a-TRAP (ES2575) mouse line

ABSTRACT: Transcriptional profiling of ependymal cells lining the ventricles in the central nervous system of a Dcdc2a-TRAP (ES2575) mouse line

PROVIDER: PRJNA590494 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10495502.fastq.gz	Fastqsanger.gz
	SRR10495503.fastq.gz	Fastqsanger.gz
	SRR10495504.fastq.gz	Fastqsanger.gz
	SRR10495505.fastq.gz	Fastqsanger.gz
	SRR10495506.fastq.gz	Fastqsanger.gz

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Transcriptional profiling of ependymal cells lining the ventricles in the central nervous system of a Dcdc2a-TRAP (ES2575) mouse line

Project description:TRAP translational profiling is a method that allows investigators to genetically characterize specific cell types in complex tissues such as mouse brain. Using this technique we obtained RNA-Seq data from actively translating transcripts present in ependymal cells lining the ventricles in the central nervous system of adult Dcdc2a-EGFP/Rpl10a (ES2575) mice

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Project description:Through single cell transcriptome analysis, we uncovered molecular signatures of CD133+/GFAP- ependymal (E) cells, CD133+/GFAP+ neural stem (B) cells, Dlx2+ neuroblasts (A cells), and Sox10+ oligodendrocyte progenitors (O cells) in the adult mouse forebrain neurogenic zone. prominent hub genes of the gene network unique to ependymal CD133+/GFAP- quiescent cells are enriched for receptors of angiogenic factors and immune-responsive genes. Administration of VEGF activated CD133+ ependymal stem cells lining not only the lateral, but also the 4th ventricles, and together with bFGF, elicited subsequent neural lineage differentiation and migration. Examination of 28 single cells and 4 populations of 10 cells from adult mouse forebrain neurogenic zone.

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