Project description:The aim of this study is to analyze the transcriptional effects of Aire deficiency in the thymus, using the Affymetrix MoGene platform to analyze variation in exon usage MECs were isolated from 4-6 wk-old WT or Aire KO ((B6xNOD)F1 background) mice. Three WT and three Aire-KO mice taken individually were used.

2011-11-23 | E-GEOD-33878 | biostudies-arrayexpress

WT and Aire-KO B6 MEChi RNAseq profiling

Project description:Aire-sensitive genes exhibit a weak inclusion of alternative splicing events

2022-01-07 | GSE177062 | GEO

Aire-KO MEChi RNAseq profiling

Project description:Background: In order to become functionally competent but harmless mediators of the immune system, T cells undergo a strict educational program in the thymus, where they learn to discriminate between self and non-self. This educational program is, to a large extent, mediated by medullary thymic epithelial cells (mTECs) that have a unique capacity to express, and subsequently present a large fraction of body antigens. While the scope of promiscuously expressed genes by mTECs is well established, relatively little is known about the expression of variants that are generated by co- and post-transcriptional processes. Results: Our study reveals that in comparison to other cell types, mTECs display significantly higher levels of alternative splicing, as well as A-to-I and C-to-U RNA editing, which thereby further expand the diversity of their self-antigen repertoire. Interestingly, Aire, the key mediator of mTECs promiscuous gene expression, plays a limited role in the regulation of these transcriptional processes. Conclusions: Our results highlight RNA processing as another layer by which the immune system assures a comprehensive self-representation in the thymus which is required for the establishment of self-tolerance and prevention of autoimmunity. Identification of the number of genes expressed in Aire-KO MEChi

2016-09-23 | GSE87133 | GEO

RNAseq profiling of recirculating thymic Tregs in Aire-KO and WT mice

Project description:Impact of Aire on CCR6+ Treg suppressive properties

2023-01-18 | GSE188419 | GEO

RNAseq E16.5 mouse skin Irf6 KO vs WT

Project description:RNAseq E16.5 mouse skin Irf6 KO vs WT

| PRJNA510612 | ENA

Renal transcriptome profiling by RNAseq in Bicc1 WT and KO mice.

Project description:Renal transcriptome profiling by RNAseq in Bicc1 WT and KO mice.

| PRJEB24616 | ENA

RNAseq on lungs from WT vs claudin 4 KO mice that underwent ventilator-induced lung injury

Project description:WT mice and claudin 4 KO mice were exposed to ventilator-induced lung injury (VILI) for 2 hours. We found that in some Cldn4 KO mice, injury was similar to WT, while in others, injury was higher, as assessed by amount of protein leak into broncho-alveolar lavage fluid. We performed RNAseq to find which genes were responsible for higher injury in Cldn4 KO mice. WT mice and claudin 4 KO mice were exposed to ventilator-induced lung injury (VILI) for 2 hours. RNA were extracted from whole lungs and RNA sequencing was performed. The samples are (all in duplicates): WT no VILI, Cldn4 KO no VILI, WT VILI, Cldn4 KO VILI with similar injury to WT (Cldn4 KOlow), and Cldn4 KO VILI with higher injury than WT (Cldn4 KOhigh)

2014-11-04 | E-GEOD-50927 | biostudies-arrayexpress

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