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Single-cell RNA-sequencing of tdTomato-labeled c-Kit lineage cells in aortic allografts of mouse models

ABSTRACT: Single-cell RNA-sequencing of tdTomato-labeled c-Kit lineage cells in aortic allografts of mouse models

PROVIDER: PRJNA591590 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10529453_1.fastq.gz	Fastqsanger.gz
	SRR10529453_2.fastq.gz	Fastqsanger.gz
	SRR10529454_1.fastq.gz	Fastqsanger.gz
	SRR10529454_2.fastq.gz	Fastqsanger.gz

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Single-cell RNA-sequencing of tdTomato-labeled c-Kit lineage cells in aortic allografts of mouse models

Project description:We reported the transcriptional profiling of tdTomato-labeled c-Kit lineage cells in a mouse model of transplant arteriosclerosis by single-cell RNA sequencing.

2020-09-08 | GSE140968 | GEO

Single-cell RNA-sequencing of aortic allografts in mouse models

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Next Generation Sequencing Facilitates Quantitative Analysis of mock oligodendrocytes (Ols) and tdTomato+ and tdTomato- Ols Transcriptomes.

Project description:Purpose: Comparision transcriptomes of isolated tdTomato+ and tdTomato- OLs from the brains and spinal cords of infected mice at 30 dpi to those of OLs isolated from mock-infected central nervous system (CNS) samples by nextgen sequencing Methods: Brains and spinal cords were harvested at day 30 p.i. and processed as described above. After Live/Dead staining and treatment with Fc block, OLs were identified using APC-anti-O4 (1:50; Miltenyi). RNA was extracted from sorted OLs using an RNeasy Micro Kit (Qiagen). RNA sequence analysis was performed at the University of Minnesota Genomics Center. Results: We identified 158 and 115 genes, that showed a significant difference (p<0.05) when tdTomato+ and tdTomato- cells were compared to cells from mock-infected mice, respectively, with 61 mRNAs common to both tdTomato- and tdTomato+ cells

2020-04-15 | GSE148650 | GEO

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