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Genetic and microstructural differences in the cortical plate of gyri and sulci during gyrification in fetal sheep

ABSTRACT: Genetic and microstructural differences in the cortical plate of gyri and sulci during gyrification in fetal sheep

PROVIDER: PRJNA592896 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10573373.fastq.gz	Fastqsanger.gz
	SRR10573374.fastq.gz	Fastqsanger.gz
	SRR10573375.fastq.gz	Fastqsanger.gz
	SRR10573376.fastq.gz	Fastqsanger.gz
	SRR10573377.fastq.gz	Fastqsanger.gz

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Similar Datasets

Genetic and microstructural differences in the cortical plate of gyri and sulci during gyrification in fetal sheep

Project description:Gyrification of the cerebral cortex is a developmentally important process, however the mechanisms that drive cortical folding remain unknown. Theories propose that changes within the cortical plate (CP) cause gyrification, yet differences between the CP below gyri and sulci have not been investigated. Here we report genetic and microstructural differences in the CP below gyri and sulci assessed before (gestational age (GA) 70), during (GA 90) and after (GA 110) gyrification in fetal sheep. The areal density of BDNF-, CDK5-, and NeuroD6-immunopositive cells were increased, and HDAC5 and MeCP2 mRNA levels were decreased, in the CP below gyri compared to sulci during gyrification, but not before. Only the areal density of BDNF-immunopositive cells remained increased after gyrification. MAP2-immunoreactivity was also increased in the CP below gyri compared to sulci at GA 90, and this was associated with microstructural changes assessed via neurite orientation dispersion and density imaging (NODDI) at GA 98. Differential neurite outgrowth may therefore be a strong candidate to explain local changes in CP architecture during gyrification.

2019-12-03 | GSE141233 | GEO

Expression data from fetal sheep immunocytes

Project description:study investigating the initiation of systemic inflammatory signaling in fetuses exposed to TLR-4 agonist lipopolysaccharides from E.coli microarray was used to determine the inflammatory transcriptome in circulating fetal immunocytes fetal sheep at 92d GA were catheterised prior to intraamniotic exposure fo saline or lipopolysaccharides for 48h prior to euthanasia

2015-08-21 | E-GEOD-72240 | biostudies-arrayexpress

Transplanting FVIII/ET3-Secreting Cells in Fetal Sheep Increases FVIII Levels Long-Term Without Inducing Immunity or Toxicity

Project description:To investigate the differentiative fate of human PLCs following transplantation into fetal sheep and engraftment in various tissues/organs, we performed gene expression profiling analysis using data obtained from RNA-seq of human PLCs prior to in utero transplantation and of each engrafted fetal sheep tissue after filtering to remove any cross-reactivity with orthologous sheep transcripts

2023-06-01 | GSE230081 | GEO

Single cell RNAseq analysis of murine fetal growth plate chondrocytes and perichondrial cells

Project description:We revealed a single cell transcriptional landscape of fetal growth plate chondrocytes and perichondrial cells.

2019-02-26 | GSE126966 | GEO

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map

Project description:The cortical area map is initially patterned by transcription factor (TF) gradients in the neocortical primordium, which define a protomap in the embryonic ventricular zone (VZ). However, mechanisms that propagate regional identity from VZ progenitors to cortical plate (CP) neurons are unknown. Here we show that the VZ, subventricular zone (SVZ), and CP contain distinct molecular maps of regional identity, reflecting different gene expression gradients in radial glia progenitors, intermediate progenitors, and projection neurons, respectively. The intermediate map in SVZ is modulated by Eomes (also known as Tbr2), a T-box TF. Eomes inactivation caused rostrocaudal shifts in SVZ and CP gene expression, with loss of corticospinal axons and gain of corticotectal projections. These findings suggest that cortical areas and connections are shaped by sequential maps of regional identity, propagated by the Pax6 ? Eomes ? Tbr1 TF cascade. In humans, PAX6, EOMES, and TBR1 have been linked to intellectual disability and autism. To determine the role of Eomes in the propagation of the protomap to cortical plate neurons, used microarray analysis of E14.5 cortex from five wild type and three Eomes knockout mice.

2013-04-02 | E-GEOD-43387 | biostudies-arrayexpress

Single cell RNAseq analysis of fetal growth plate-derived bone marrow mesenchymal stromal cells

Project description:We revealed a single cell transcriptional landscape of bone marrow mesenchymal stromal cells derived from the fetal growth plate cartilage

2019-02-26 | GSE126968 | GEO

Effects of Maternal Energy Intake on Genomic Imprinting in Fetal Sheep

Project description:Background: Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parental origin. To date a total of 255 imprinted genes have been identified or predicted among all investigated mammal species. However, only 21 have been described in sheep and 11 are annotated in current ovine genome. Results: Here we aim to use DNA/RNA throughput sequencing to identify monoallelically expressed and imprinted genes in organs of day 135 fetal sheep, and 2) to determine whether different levels of maternal energy intake (100% of NRC energy requirement or control, 140% or over-, and 60% or under-fed) influence genomic imprinting. We report strategies to solve technical challenges in the next-generation sequencing data analysis pipeline, including alignment bias of RNA sequencing reads and filtering potential false positives. We identified 80 monoallelically expressed and 18 putatively imprinted genes using the list of 255 stated above as a guide. Five (45.6% of 11) were already known imprinted genes in sheep, the other 13 were known imprinted in other mammals. Sanger sequencing confirmed four putative sheep imprinted genes INPP5F, PLAGL1, CASD1 and PPP1R9A. Among the 13 putative imprinted genes, five localized in the known sheep imprinting clusters of MEST domain on chromosome 4, DLK1/GTL2 domain on chromosome 18 and IGF2/H19 domain on chromosome 21, three were in a novel sheep imprinted cluster on chromosome 4 known in other species as PEG10/SGCE. Additionally, we found that the imprinted genes PHLDA2, SLC22A18, DIRAS3, and IGF2 were differentially expressed, albeit without allelic expression reversal, among the three maternal nutritional groups. Conclusions: Together, our results expanded the sheep imprinted gene list to 34 and demonstrated the influence of maternal diet on fetal imprinting under the conditions studied.

2018-08-29 | GSE111306 | GEO

Lipidomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-II)

Project description:Cardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis

2018-09-10 | ST001061 | MetabolomicsWorkbench

NMR Metabolomics of Near-Term Fetal and Newborn Sheep Cardiac Tissue (part-I)

Project description:Cardiac tissue from near-term fetal and newborn sheep were compared via NMR metabolomic analysis

2018-08-31 | ST001060 | MetabolomicsWorkbench

Exploring the transcriptomic pattern in two kind of sheep skin with remarkable phenotype differences on wool

Project description:We report the application of High throughout sequencing to explore the differences of skin between Super Merino sheep(SM) and Small Tail Han sheep, which have remarkable phenotype differences on wool and hair follicle traits. We analysed the expression data by CLC genomic workbench 9.0 software. We find there are 435 differential expressional genes (DEGs) (127 were up-regulated and 308 were down-regulated) when STH sheep as control group. Some hair follicle KRTs, KAPs genes and hair follicle stem cells marker genes, were up-regulated in SM sheep. However, some of mammalian epidermal development complex (EDC) family genes were up-regulated in STH sheep. The GO and gene network analysis shown high expression genes in SM sheep enriched on type I interferon, lipid/fatty acid synthesis metabolism. This study provide more details in skin which control the development of follicle and wool in sheep.

2017-11-16 | GSE95785 | GEO

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