Project description:Purpose: To identify gene expression changes in CCK neurons of hippocsmpus of SMARCA3 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from CCK+ cells and, cDNA synthesis and next generation RNAseq using Illumina Nextseq sequencer. Results: Biostatistical analysis identified 1378 genes that were altered by SMARCA3 cKO.
Project description:Purpose: To identify gene expression changes in cholinergic neurons of the ventral striatum of p11 cKO mice Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from ChAT+ cells and, cDNA synthesisi and next generation RNAseq using Illumina Hiseq sequencer. Results: Biostatistical analysis identified 113 genes that were altered by p11. 59 genes were up-regulated and 54 down-regulated.
Project description:Purpose: To identify chromatin accessibility changes in CCK hippocampal interneurons from SMARCA3 cKO mice Method: Genetically expressing GFP nuclei were sorted from CCK+ or GAD2+ cells from WT or SMARCA3 cKO mice, and were used for ATAC seq using Nextseq sequencer. Results: Biostatistical analysis identified 3552 genes that were altered by SMARCA3 cKO in CCK cells.
Project description:Fundic mucosa gene expression in wildtype vs gastrin knockout, gastrin replaced gastrin KO and gastrin-CCK double knockout mice; Fundic mucosal scraping RNA were isolated from WT, Gastrin Knockout, Gastrin replaced KO and Gastrin-CCK double knockout mice. Targets from three biological replicates of each were generated and the expression profiles were determined using Affymetrix murine 430A arrays. Comparisons between the sample groups allow the identification of genes with gastrin responsiveness and Gastrin-CCK double effect . Experiment Overall Design: 3 WT, 3 Gastrin KO, 3 Gastrin replaced KO and 3 Gastrin-CCK double Knockout fundic RNA replicates were analyzed