Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

ABSTRACT: CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

PROVIDER: PRJNA593610 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10588839_1.fastq.gz	Fastqsanger.gz
	SRR10588839_2.fastq.gz	Fastqsanger.gz
	SRR10588840_1.fastq.gz	Fastqsanger.gz
	SRR10588840_2.fastq.gz	Fastqsanger.gz
	SRR10588841_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 56

Similar Datasets

CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

Project description:Purpose: The goals of this study are to compare NGS-derived total transcriptome profiling (RNA-seq) of CNOT1-depleted cells (using siRNA) with control HeLa cells before and after DNA damage to examine quantitative gene expression.

2019-12-05 | GSE141496 | GEO

The genomic and transcriptomic landscape of a HeLa cell line.

Project description:HeLa is the most widely used model cell line for studying human cellular and molecular biology. To date, no genomic reference for this cell line has been released, and experiments have relied on the human reference genome. Effective design and interpretation of molecular genetic studies performed using HeLa cells require accurate genomic information. Here we present a detailed genomic and transcriptomic characterization of a HeLa cell line. We performed DNA and RNA sequencing of a HeLa Kyoto cell line and analyzed its mutational portfolio and gene expression profile. Segmentation of the genome according to copy number revealed a remarkably high level of aneuploidy and numerous large structural variants at unprecedented resolution. Some of the extensive genomic rearrangements are indicative of catastrophic chromosome shattering, known as chromothripsis. Our analysis of the HeLa gene expression profile revealed that several pathways, including cell cycle and DNA repair, exhibit significantly different expression patterns from those in normal human tissues. Our results provide the first detailed account of genomic variants in the HeLa genome, yielding insight into their impact on gene expression and cellular function as well as their origins. This study underscores the importance of accounting for the strikingly aberrant characteristics of HeLa cells when designing and interpreting experiments, and has implications for the use of HeLa as a model of human biology.

| S-EPMC3737162 | biostudies-literature

Ribosome Profiling with CNOT1 depletion

Project description:Ribosome profiling in HEK293 cells with and without the depletion of CNOT1. This allows the determination of translational efficiency and nucleotide resolution of ribosome occupancy.

2021-08-23 | GSE158141 | GEO

The genomic Landscape of an HeLa cell line.

Project description:Genotyping by high-throughput sequencing of HeLa Kyoto H2B-mRFP/mEGFP-alpha-tubulin cell line

| PRJEB426 | ENA

Ribosome Profiling with CNOT1 depletion

Project description:Ribosome Profiling with CNOT1 depletion

| PRJNA664116 | ENA

Homo sapiens cultivar:HeLa cell line

Project description:Homo sapiens cultivar:HeLa cell line Raw sequence reads

| PRJNA435660 | ENA

EWS siRNA knock-down in HeLa cell line

Project description:HeLa cell line was trasfected by a EWS siRNA oligo causing knock-down of EWS or a siRNA scrambled oligo