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Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [DropSeq]

ABSTRACT: Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [DropSeq]

PROVIDER: PRJNA594860 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10668487_1.fastq.gz	Fastqsanger.gz
	SRR10668487_2.fastq.gz	Fastqsanger.gz
	SRR10668488_1.fastq.gz	Fastqsanger.gz
	SRR10668488_2.fastq.gz	Fastqsanger.gz

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Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [DropSeq]

Project description:Using DropSeq single cell RNA sequencing, we report that neuronal derived-IL-18 is required for goblet cell expression of intestinal antimicrobial protein expression Mucosal barrier immunity is essential for the maintenance of the commensal microflora and combating invasive bacterial infection. Although immune and epithelial cells are thought to be the canonical orchestrators of this complex equilibrium, here we show that the enteric nervous system (ENS) plays an essential and non-redundant role in governing the anti-microbial protein (AMP) response. Using confocal microscopy and single-molecule fluorescence in situ mRNA-hybridization (smFISH) studies, we observed that intestinal neurons produce the pleiotropic cytokine IL-18. Strikingly, deletion of IL-18 from the enteric neurons alone, but not immune or epithelial cells, rendered mice susceptible to invasive Salmonella typhimurium (S.t.) infection. Mechanistically, unbiased RNA sequencing and single cell sequencing revealed that enteric neuronal IL-18 is specifically required for homeostatic goblet cell AMP production. Together, we show that neuron derived IL-18 signaling controls tissue wide intestinal immunity and has profound consequences on the mucosal barrier and invasive bacterial killing.

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Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity

Project description:Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity

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Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [RNA-seq]

Project description:Using RNA sequencing, we report that neuron derived-IL-18 is required for intestinal antimicrobial protein expression Mucosal barrier immunity is essential for the maintenance of the commensal microflora and combating invasive bacterial infection. Although immune and epithelial cells are thought to be the canonical orchestrators of this complex equilibrium, here we show that the enteric nervous system (ENS) plays an essential and non-redundant role in governing the anti-microbial protein (AMP) response. Using confocal microscopy and single-molecule fluorescence in situ mRNA-hybridization (smFISH) studies, we observed that intestinal neurons produce the pleiotropic cytokine IL-18. Strikingly, deletion of IL-18 from the enteric neurons alone, but not immune or epithelial cells, rendered mice susceptible to invasive Salmonella typhimurium (S.t.) infection. Mechanistically, unbiased RNA sequencing and single cell sequencing revealed that enteric neuronal IL-18 is specifically required for homeostatic goblet cell AMP production. Together, we show that neuron derived IL-18 signaling controls tissue wide intestinal immunity and has profound consequences on the mucosal barrier and invasive bacterial killing.

2019-12-12 | GSE141808 | GEO

Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [RNA-seq]

Project description:Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity [RNA-seq]

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Enteric Nervous System-Derived IL-18 Orchestrates Mucosal Barrier Immunity.

Project description:Mucosal barrier immunity is essential for the maintenance of the commensal microflora and combating invasive bacterial infection. Although immune and epithelial cells are thought to be the canonical orchestrators of this complex equilibrium, here, we show that the enteric nervous system (ENS) plays an essential and non-redundant role in governing the antimicrobial protein (AMP) response. Using confocal microscopy and single-molecule fluorescence in situ mRNA hybridization (smFISH) studies, we observed that intestinal neurons produce the pleiotropic cytokine IL-18. Strikingly, deletion of IL-18 from the enteric neurons alone, but not immune or epithelial cells, rendered mice susceptible to invasive Salmonella typhimurium (S.t.) infection. Mechanistically, unbiased RNA sequencing and single-cell sequencing revealed that enteric neuronal IL-18 is specifically required for homeostatic goblet cell AMP production. Together, we show that neuron-derived IL-18 signaling controls tissue-wide intestinal immunity and has profound consequences on the mucosal barrier and invasive bacterial killing.

| S-EPMC7339937 | biostudies-literature

Enteric Nervous System Derived IL-18 Orchestrates Mucosal Barrier Immunity

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-12-12 | GSE141810 | GEO

RNA Expression Data from developing enteric nervous system (ENS) and bowel

Project description:The enteric nervous system (ENS) can control most essential gut functions owing to its organization into complete neural circuits consisting of a multitude of different neuronal subtypes. We used microarrays to identify transcription factor networks and signaling pathways involved in diversification and differentiation of enteric neurons during development of the enteric nervous system.

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2015-09-01 | E-GEOD-72560 | biostudies-arrayexpress

Lineage-dependent spatial and functional organisation of the mammalian enteric nervous system

Project description:Single-cell transcriptomic analysis to delineate key regulators of neurogenic commitment in the enteric nervous system. To resolve the molecular profiles of ENCCs and identify transcriptional signatures of lineage restriction and differentiation, we analyzed their transcriptomes by single-cell RNA-sequencing (scRNA-seq). E12.0 Sox10::CreERT2;Rosa26tdTomato (SER93|tdT) embryos were exposed to a single dose of TM (100g/g of dam body weight) and tdT+ gut cells were isolated 20-24 hours later by flow cytometry.

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