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Transcriptome analysis of hIPSCs differentiation towards the skeletal muscle and motor neurons lineages

ABSTRACT: Transcriptome analysis of hIPSCs differentiation towards the skeletal muscle and motor neurons lineages

PROVIDER: PRJNA595642 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10692960_1.fastq.gz	Fastqsanger.gz
	SRR10692960_2.fastq.gz	Fastqsanger.gz
	SRR10692961_1.fastq.gz	Fastqsanger.gz
	SRR10692961_2.fastq.gz	Fastqsanger.gz
	SRR10692962_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of hIPSCs differentiation towards the skeletal muscle and motor neurons lineages

Project description:We report the application of RNA sequencing technology for high-throughput expression profiling in human Induced Pluripotent Stem cells differentiated toward skeletal muscle and motor neurons lineages. Transcriptomic analysis was performed at different time points during the differentiation process

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Cardiopharyngeal Mesoderm specification into cardiac and skeletal muscle lineages in gastruloids

Project description:Cardiopharyngeal mesoderm contributes to the formation of the heart and head muscles. However, the mechanisms governing cardiopharyngeal mesoderm specification remain unclear. Indeed, there is a lack of an in vitro model replicating the differentiation of both heart and head muscles to study these mechanisms. Such models are required to allow live-imaging and high throughput genetic and drug screening. Here, we show that the formation of self-organizing or pseudo-embryos from mouse embryonic stem cells (mESCs), also called gastruloids, reproduces cardiopharyngeal mesoderm specification towards cardiac and skeletal muscle lineages. By conducting a comprehensive temporal analysis of cardiopharyngeal mesoderm establishment and differentiation in gastruloids and comparing it to mouse embryos, we present the first evidence for skeletal myogenesis in gastruloids. By inferring lineage trajectories from the gastruloids single-cell transcriptomic data, we further suggest that heart and head muscles formed in gastruloids derive from cardiopharyngeal mesoderm progenitors. We identify different subpopulations of cardiomyocytes and skeletal muscles, which most likely correspond to different states of myogenesis with “head-like” and “trunk-like” skeletal myoblasts. These findings unveil the potential of mESC-derived gastruloids to undergo specification into both cardiac and skeletal muscle lineages, allowing the investigation of the mechanisms of cardiopharyngeal mesoderm differentiation in development and how this could be affected in congenital diseases.

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Proteomic profiling of enteroid cultures skewed towards development of specific epithelial lineages

Project description:Recently, three-dimensional small intestinal organoids (enteroids) have been developed from cultures of intestinal stem cells which differentiate in vitro to generate all the differentiated epithelial cell types associated with the intestine and mimic the structural properties of the intestine observed in vivo. Small-molecule drug treatment can skew organoid epithelial cell differentiation towards particular lineages, and these skewed enteroids may provide useful tools to study specific epithelial cell populations, such as goblet and Paneth cells. However, the use and characterisation of enteroid models has not yet been fully explored, such that the extent to which differentiated epithelial cell populations in these skewed enteroids represent their in vivo counterparts is not fully understood. In this study, we have performed label-free quantitative proteomics to determine whether skewing murine enteroid cultures towards the goblet or Paneth cell lineages results in changes in abundance of proteins associated with these cell lineages in vivo. Our data confirm that skewed enteroids recapitulate important features of the in vivo gut environment, confirming that they can serve as useful models for the investigation of normal and disease processes in the intestine. Furthermore, by comparison of our mass spectrometry data with histology data contained within the Human Protein Atlas, we identify putative novel markers for goblet and Paneth cells.

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Global expression profile difference of hiPSCs between hESCs and human Urine-Derived Cells

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Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages

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