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Analysis of the turkey reproductive tract transcriptome

ABSTRACT: Analysis of the turkey reproductive tract transcriptome

PROVIDER: PRJNA597008 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10746605_1.fastq.gz	Fastqsanger.gz
	SRR10746605_2.fastq.gz	Fastqsanger.gz
	SRR10746606_1.fastq.gz	Fastqsanger.gz
	SRR10746606_2.fastq.gz	Fastqsanger.gz
	SRR10746607_1.fastq.gz	Fastqsanger.gz

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Analysis of the turkey reproductive tract transcriptome

Project description:The male reproductive tract tissue samples were obtained from six 38-wk-old turkeys. The gene expression pattern in turkey testis, epididymis and ductus deferens were determined by high-throughput transcriptome sequencing. The obtained sequence reads were mapped to the turkey genome, and relative expression values were calculated for the analysis of differential expressed genes. Bioinformatics analysis revealed several candidate genes potentially involved in spermatogenesis, spermiogenesis and flagella formation in testis and post-testicular sperm maturation in epididymis and ductus deferens. The achievement of spermatozoa motility during post-testicular maturation were found to be linked with development of flagellum actin filaments and biochemical processes including Ca2+ influx and protein phosphorylation/dephosphorylation. Finally, genes involved in reproductive system development and morphogenesis were identified.

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Project description:The development of a safe and effective, reversible, non-hormonal contraceptive method for men has been an ongoing effort for the past few decades, yet none have reached clinical trials. To advance progress, further identification of novel reproductive tract-specific, potentially druggable protein targets is imperative. In this study, we expand on previous single tissue, single species studies by integrating analysis of a vast number of publicly available human and mouse RNA- seq datasets whose initial published purpose was not focused on identifying male reproductive tract-specific targets. We also incorporate analysis of additional newly acquired human and mouse testis and epididymis samples to increase the number of targets identified. Enclosed in this accession is the RNA-seq sequence data for the 9 new mouse epididymis segment samples we processed for analysis.

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