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ABSTRACT: DNA methylation data

PROVIDER: PRJNA598317 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR10808207_1.fastq.gz	Fastqsanger.gz
	SRR10808207_2.fastq.gz	Fastqsanger.gz
	SRR10808208_1.fastq.gz	Fastqsanger.gz
	SRR10808208_2.fastq.gz	Fastqsanger.gz
	SRR10808209_1.fastq.gz	Fastqsanger.gz

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Project description:Methylation of cytosines (5meC) is a widespread heritable DNA modification. During mammalian development, two global demethylation events are followed by waves of de novo DNA methylation. In vivo mechanisms of DNA methylation establishment are largely uncharacterized. Here we use Saccharomyces cerevisiae as a system lacking DNA methylation to define the chromatin features influencing the activity of the murine DNMT3B. Our data demonstrate that DNMT3B and H3K4 methylation are mutually exclusive and that DNMT3B is co-localized with H3K36 methylated regions. In support of this observation, DNA methylation analysis in yeast strains without Set1 and Set2 show an increase of relative 5meC levels at the TSS and a decrease in the gene-body, respectively. We extend our observation to the murine male germline, where H3K4me3 is strongly anti-correlated while H3K36me3 correlates with accelerated DNA methylation. These results show the importance of H3K36 methylation for gene-body DNA methylation in vivo. Collecting Yeast Whole Genome Bisulfite Sequencing Data

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