Project description:Human cancer cell lines (DLD1 wt or ZNF692 KO, and for IP-proteomics HCT116 transfected with GFP, GFP-ZNF692 and deltaNolsZNF692). 788570, HCT116 transfected with GFP;
788571, HCT116 transfected with GFP-ZNF692;
788572, HCT116 transfected with deltaNolsZNF692;
937612, control 40S subunit from DLD1 cells;
937613, control 60S subunit from DLD1 cells;
937614, control 80S monosome fraction from DLD1 cells;
937615, KO ZNF692 40S subunit from DLD1 cells;
937616, KO ZNF692 60S subunit from DLD1 cells;
937617, KO ZNF692 80S monosome fraction from DLD1 cells;
943031, control 40S subunit from DLD1 cells;
943032, control 60S subunit from DLD1 cells;
943033, control 80S monosome fraction from DLD1 cells;
943034, KO ZNF692 40S subunit from DLD1 cells;
943035, KO ZNF692 60S subunit from DLD1 cells;
943036, KO ZNF692 80S monosome fraction from DLD1 cells
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to use RNA-seq to find differences between WT and MTF1-KO tumor in colorectal cancer Methods: HCT116 cells and MTF1-KO HCT116 cells were injected subcutaneously into the back of NSG mice at 2x106 cells/recipient , At day 24 after injection,Tumor tissue were isolated from infected recipients,and Total RNA was collected. Besides,about 5x106 cultured HCT116 cells and MTF1-KO HCT116 cells were collected for total RNA isolation ,and sent all of them for sequencing. Conclusions: Our study represents the transcriptome difference between WT HCT116 tumor and MTF1-KO HCT116 tumor has a very positive significance for the pathological process of colorectal cancer,especially for cell adhension
Project description:Genome-wide DNA methylation profiling of HCT116 WT, HCT116 DNMT1 and DNMT3B double KO, and breast cancer tumors by next generation Infinium assay
Project description:Genome-wide DNA methylation profiling of HCT116 WT, HCT116 DNMT1 and DNMT3B double KO, and breast cancer tumors by next generation Infinium assay Bisulfite converted DNA from 22 samples were hybridized to the Illumina's HumanMethylation450 BeadChip
Project description:FBXW7 modulates stress response by post-translational modification of HSF1 HSF1 orchestrates the heat-shock response upon exposure to heat stress and activates a transcriptional program vital for cancer cells. In this study we assayed for genome-wide localization of HSF1 enrichment in the HCT116 FBXW7 KO colon cells and their wild type counterpart in untreated cells and upon heat shock. These results revealed that accumulation of nuclear HSF1 in FBXW7 KO cells results in rewiring of the HSF1 transcriptional program. Five million cells were used for the ChIP and precipitated using 5 micrograms of antibody (cell signaling, 4356) against human HSF1