Project description:Human cancer cell lines (DLD1 wt or ZNF692 KO, and for IP-proteomics HCT116 transfected with GFP, GFP-ZNF692 and deltaNolsZNF692). 788570, HCT116 transfected with GFP;
788571, HCT116 transfected with GFP-ZNF692;
788572, HCT116 transfected with deltaNolsZNF692;
937612, control 40S subunit from DLD1 cells;
937613, control 60S subunit from DLD1 cells;
937614, control 80S monosome fraction from DLD1 cells;
937615, KO ZNF692 40S subunit from DLD1 cells;
937616, KO ZNF692 60S subunit from DLD1 cells;
937617, KO ZNF692 80S monosome fraction from DLD1 cells;
943031, control 40S subunit from DLD1 cells;
943032, control 60S subunit from DLD1 cells;
943033, control 80S monosome fraction from DLD1 cells;
943034, KO ZNF692 40S subunit from DLD1 cells;
943035, KO ZNF692 60S subunit from DLD1 cells;
943036, KO ZNF692 80S monosome fraction from DLD1 cells
Project description:Proteomics on HCT116 cells. 3 samples wild-type, 3 samples Fbw7 KO. Subcellular fractionation to cytoplasmic and nuclear fractions. Proteins were reduced with DTT, alkylated with iodoacetamide, digested with trypsin, and labelled with TMT6-plex: 126,127,128 - wildtype; 129,130,131 - Fbw7 KO. Sample separation by high resolution isoelectric focusing at peptide level, whereafter 72 fractions were loaded onto reversed phase LC-MSMS
Project description:The goal of this study was to understand how PHRF1 regulates gene expression in HCT116 cells. By conducting RNA sequencing of 3 WT and 3 KO (PHRF1 knockout) samples, we found that DNA damage response pathways were upregulated and splicing-related pathways were downregulated in KO cells versus WT cells.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to use RNA-seq to find differences between WT and MTF1-KO tumor in colorectal cancer Methods: HCT116 cells and MTF1-KO HCT116 cells were injected subcutaneously into the back of NSG mice at 2x106 cells/recipient , At day 24 after injection，Tumor tissue were isolated from infected recipients，and Total RNA was collected. Besides,about 5x106 cultured HCT116 cells and MTF1-KO HCT116 cells were collected for total RNA isolation ,and sent all of them for sequencing. Conclusions: Our study represents the transcriptome difference between WT HCT116 tumor and MTF1-KO HCT116 tumor has a very positive significance for the pathological process of colorectal cancer，especially for cell adhension
Project description:Gene expression profiles were obtained via Nanostring nCounter Expression Assay (PanCancer Progression Panel, Nanostring Technologies, Hamburg, Germany). We aimed to obtain a gene expression signature associated to the kockout of p21 (i.e. Cyclin Dependent Kinase Inhibitor 1A, CDKN1A) in colorectal carcinoma samples and its association with of epithelial-mesenchymal transition (EMT). We analysed and compared three independent cultures of HCT116 p21 wt cells and three HCT116 p21-/- ko cells.
Project description:Genome-wide DNA methylation profiling of HCT116 WT, HCT116 DNMT1 and DNMT3B double KO, and breast cancer tumors by next generation Infinium assay Bisulfite converted DNA from 22 samples were hybridized to the Illumina's HumanMethylation450 BeadChip
