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RNA-seq of mouse heart samples from Alginate oligosaccharides and/or busulfan treatment groups

ABSTRACT: RNA-seq of mouse heart samples from Alginate oligosaccharides and/or busulfan treatment groups

PROVIDER: PRJNA599390 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10843562_1.fastq.gz	Fastqsanger.gz
	SRR10843562_2.fastq.gz	Fastqsanger.gz
	SRR10843563_1.fastq.gz	Fastqsanger.gz
	SRR10843563_2.fastq.gz	Fastqsanger.gz
	SRR10843564_1.fastq.gz	Fastqsanger.gz

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RNA-seq of mouse heart samples from Alginate oligosaccharides and/or busulfan treatment groups

Project description:The goat of this project is to exploreAlginate oligosaccharides (AOS) effect on the recovery of heart function. RNA-seq of heart samples from different groups: Con, busulfan(B), AOS, B+AOS.

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RNA-seq of mouse lung samples from Alginate oligosaccharides and/or busulfan treatment groups

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RNA-seq of mouse spleen samples from Alginate oligosaccharides and/or busulfan treatment groups

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RNA-seq of mouse kidney samples from Alginate oligosaccharides and/or busulfan treatment groups

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Single-cell RNA-seq of the intestine sample of ICR mice treated with busulfan and/or Alginate Oligosaccharides

Project description:We profiled a single-cell RNA-seq on ICR mouse intestine treated with busulfan and or Alginate Oligosaccharides (AOS) from 3 weeks of age to 5 weeks of age (for 2 weeks) to study the rescue effect of AOS on the intestine development disrupted by busulfan. We collected single cell from intestine from the four groups AOS 0 (control), B+A 0 (busulfan alone), B+A 10 (busulfan plus AOS 10 mg/kg Body Weight). The the cells were further captured with 10x Genomics based single-cell system. By using single-cell RNA seq, we found busulfan damaged intestinal cells. AOS significantly improved the intestinal cell junction and microvilin formation. Besides, we provide candidate master regulons regulated by AOS. Our data here offers the opportunity for improving intestine under stress.

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Project description:We profiled a single-cell RNA-seq on ICR mouse testis treated with busulfan and or Alginate Oligosaccharides (AOS) from 3 weeks of age to 8 weeks of age (for 5 weeks) to study the rescue effect of AOS on the spermatogenesis disrupted by busulfan. We collected single cell from testis from the four groups AOS 0 (control), AOS 10 (AOS 10 mg/kg body weight (BW)), B+A 0 (busulfan alone), B+A 10 (busulfan plus AOS 10 mg/kg BW). The the cells were further captured with 10x Genomics based single-cell system. By using single-cell RNA seq, we found busulfan disrupted spermatogenesis by the dramatical decrease in germ cells. AOS significantly increased the number of germ cells and also promoted the germ cell development. Besides, we provide candidate master regulons regulated by AOS. Our data here offers the opportunity for treating of azoospermia or oligospermia.

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RNA-seq of mouse liver samples from Alginate oligosaccharides and/or busulfan treatment groups

Project description:RNA-seq of mouse liver samples from Alginate oligosaccharides and/or busulfan treatment groups

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RNA-seq of mouse kidney samples from Alginate oligosaccharides and/or busulfan treatment groups

Project description:RNA-seq of mouse kidney samples from Alginate oligosaccharides and/or busulfan treatment groups

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RNA-seq of mouse spleen samples from Alginate oligosaccharides and/or busulfan treatment groups

Project description:RNA-seq of mouse spleen samples from Alginate oligosaccharides and/or busulfan treatment groups

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