Ontology highlight
ABSTRACT:
PROVIDER: PRJNA599606 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR10849952_1.fastq.gz | Fastqsanger.gz | |||
| SRR10849952_2.fastq.gz | Fastqsanger.gz | |||
| SRR10849953_1.fastq.gz | Fastqsanger.gz | |||
| SRR10849953_2.fastq.gz | Fastqsanger.gz | |||
| SRR10849954_1.fastq.gz | Fastqsanger.gz |
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Project description:<p>SCFAs are the main microbial fermentation products from dietary fibers in colon and play a key role in keeping healthy in the whole-body. However, differences of SCFAs concentration in the serum and colon samples collected from rats had attracted few attentions. Here, we optimized the extract and analysis methods for quantification of ten SCFAs in both serum and colon content samples. Methanol and acetonitrile were chosen for extraction of SCFAs from serum and colon content samples, respectively. Biological samples collected from Alzheimer's disease rats treated by extract of <em>Schisandra chinensis</em> (Turcz.) Baill (SC-extract) were taken as research objects. The results showed that, the relative peak intensities of SCFAs in colon content from all groups were quite similar, and the trend was identical in serum samples. Compared with the values in human, the ratio of ten SCFAs in rat's colon was similar, while the percent of acetate in rat's serum was significantly higher. For therapy of AD, SC-extract decreased the concentration of butyrate, 3-Methyvalerate and caproate in serum samples towards the trend of normal rats. The present study may help to understand the transport of SCFAs across colonic epithelium in healthy and diseased organisms.</p><p><br></p><p><strong>SCFA serum and colon content determination</strong> is reported in the current study <strong>MTBLS2088</strong></p><p><strong>Method validation</strong> is reported in <a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS2146/' rel='noopener noreferrer' target='_blank'><strong>MTBLS2146</strong></a></p>
2020-11-24 | MTBLS2088 | MetaboLights
Project description:<p>SCFAs are the main microbial fermentation products from dietary fibers in colon and play a key role in keeping healthy in the whole-body. However, differences of SCFAs concentration in the serum and colon samples collected from rats had attracted few attentions. Here, we optimized the extract and analysis methods for quantification of ten SCFAs in both serum and colon content samples. Methanol and acetonitrile were chosen for extraction of SCFAs from serum and colon content samples, respectively. Biological samples collected from Alzheimer's disease rats treated by extract of <em>Schisandra chinensis</em> (Turcz.) Baill (SC-extract) were taken as research objects. The results showed that, the relative peak intensities of SCFAs in colon content from all groups were quite similar, and the trend was identical in serum samples. Compared with the values in human, the ratio of ten SCFAs in rat's colon was similar, while the percent of acetate in rat's serum was significantly higher. For therapy of AD, SC-extract decreased the concentration of butyrate, 3-Methyvalerate and caproate in serum samples towards the trend of normal rats. The present study may help to understand the transport of SCFAs across colonic epithelium in healthy and diseased organisms.</p><p><br></p><p><strong>Method validation</strong> is reported in the current study <strong>MTBLS2146</strong></p><p><strong>SCFA serum and colon content determination</strong> is reported in study <a href='https://www.ebi.ac.uk/metabolights/editor/study/MTBLS2088/' rel='noopener noreferrer' target='_blank'><strong>MTBLS2088</strong></a></p>
2020-11-24 | MTBLS2146 | MetaboLights
Project description:Schisandra chinensis fruit extract (SCE) and its active ingredient Schisandrin B (SchB), which are effective in the treatment of vascular diseases, have been known to suppress transforming growth factor β1 (TGF-β1)-mediated Smad activation and myosin light chain (MLC) phosphorylation in vascular smooth muscle cells (VSMCs). However, it is still largely unknown about the pharmacologic effects and mechanisms of SCE and SchB on TGF-β1-induced intracellular signaling pathways in vascular smooth muscle cells (VSMCs).
2017-12-20 | GSE87439 | GEO
Project description:Long distance transfers on genetic material (mRNA, miRNA, Protein, etc.) and metabolites (Hormone) between rootstock and scion is also well documented in the homo-grafting hybrids, but the process in distant-grafting is not reported where rootstock and scion belonging to different Familly. We repeatedly grafted the Vitis vinifera scion onto the Schisandra chinensis stock and got 20 interfamily vegetative hybrids ― Vitis vinifera / Schisandra chinensis (Vs) that could grow normally over 25 years. We found the phonotype leaf, internode, fruit of Vs upper the graft union transformed to intermediate stage between V. vinifera and S. chinensis, and the new traits was inherited by vegetative propagation. To expose the reason, using the simultaneous self-rooted Vitis vinifera (Vv) as control, differences of transcriptomics, miRNAs, degradome between Vv and Vs were analyzed by high-throughput sequencing, and the metabolome by liquid Chromatograph Mass Spectrometer (LC-MS). The results showed that a total of 2113 differentially expressed genes (DEGs), GO annotation and KEGG pathway enrichment analysis found that DEGs function mainly included oxidation-reduction process, metabolic process, etc. 79 differentially expressed miRNAs (DEMs) contains 27 known miRNAs and 52 novel miRNAs were identificated, the main functions of DEMs were defense response, biosynthetic process, etc. The target gene detected by degradome sequencing were mainly regulation of transcription & DNA-templated, auxin-activated signaling pathway, etc. And the degradation group detected 840 target genes corresponding to 252 miRNAs, of which 12 DEMs and the corresponding target gene expression levels were mostly negatively correlated. Meanwhile, 1188 differentially metabolic compounds were identified, the most common of which were sputum metabolism, arginine and proline metabolism, and ABC transporters. Especially the metabolites schizandrin was significantly down regulated and gomisin was up regulated in Vs that were main medicinal ingredients in Schisandra. KEGG pathway enrichment analysis of differential metabolites found more than 30 differential metabolic pathways of Vv-Vs. Our data demonstrate the impact of interfamily graft on the genetic materials and metabolites for the scion and the traits can stable inherited by vegetative propagation, providing an important reference and basis for future breeding through select target gene type rootstocks.
2022-11-30 | GSE130209 | GEO
Project description:Schisandra chinensis Genome sequencing and assembly
| PRJNA726750 | ENA
Project description:Schisandra chinensis Transcriptome or Gene expression
| PRJNA817680 | ENA
Project description:Schisandra chinensis flower buds transcriptome sequencing
| PRJNA1027980 | ENA