Ontology highlight
ABSTRACT:
PROVIDER: PRJNA672778 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12959829_1.fastq.gz | Fastqsanger.gz | |||
| SRR12959829_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 2 of 2 |
Similar Datasets
Project description:Rheum officinale Baill. Raw sequence reads
| PRJNA1063777 | ENA
Project description:Rosa chinensis ‘Pallida’ (Rosa L.) is one of the most important ancient rose cultivars originating from China. It contributed the ‘tea scent’ trait to modern roses. However, little information is available on the gene regulatory networks involved in scent biosynthesis and metabolism in Rosa. In this study, the transcriptome of R. chinensis ‘Pallida’ petals at different developmental stages, from flower buds to senescent flowers, was investigated using Illumina sequencing technology. De novo assembly generated 89,614 clusters with an average length of 428 bp. Based on sequence similarity search with known proteins, 62.9% of total clusters were annotated. Out of these annotated transcripts, 25,705 and 37,159 sequences were assigned to gene ontology and clusters of orthologous groups, respectively. The dataset provides information on transcripts putatively associated with known scent metabolic pathways. Digital gene expression (DGE) was obtained using RNA samples from flower bud, open flower and senescent flower stages. Comparative DGE and quantitative real time PCR permitted the identification of five transcripts encoding proteins putatively associated with scent biosynthesis in roses. The study provides a foundation for scent-related genes discovery in roses.
2015-01-01 | GSE54486 | GEO
Project description:Rheum officinale Baill. Transcriptome Raw sequence reads
| PRJNA489572 | ENA
Project description:Pistacia chinensis Bunge is known as dioecious, but we have found wild monoecious individuals. In order to screen the candidate genes which may influence the sex expression or floral phenotypic differences of P. chinensis, the inflorescence buds for different sex types associated with the sex differentiation were selected and tested for small RNA sequencing. Sex-specific differentially expressed small RNA were discovered, combined with real-time PCR data, the regulation patterns of various sex types were first revealed. Our study represents the first detailed analysis of small RNA sequencing, providing more clues for understanding the mechanism of sex determination on P. chinensis.
2020-01-01 | GSE128023 | GEO
Project description:The Chinese surf clam (Mactra chinensis) is an economically important clam, distributed in Liaoning and Shandong province. In recently years，because of coastal environmental deterioration and overfishing, the natural population of M. chinensis have considerably declined . In this paper, we study the microRNA transcriptome of gills, including control and experimental group was sequenced through Illumina Hi-seq 2500 CE. And the differential expression was used to find the functional microRNA response to the Cd2+ exposure. Through Illumina Hi-seq 2500, a total of 14,415,256 clean reads and 15,570,111 clean reads were yielded in the gill of control and experimental group respectively. A total of 14,584,077 sRNA, in which there are 12,505,055 sRNA shared by S01 & S02, including 187,859 unique sRNA. The distribution of the sRNA length in the two library was similar, most of them were 26-27 nt. 27 nt was the most abundant length in S01, followded by 28 nt, 26 nt, and 23 nt; 26 nt was the most abundant length in S02, and followed by 27 nt, 28 nt and 23 nt. 50 miRNA was found in unique sRNA, including 38 conserved and 12 novel genes. The most abundant length of microRNA in the two library was the same, 23 nt. Through the analyze of differential expression analysis, the expression of 5 miRNA was induced with significantly difference, and 17 miRNA was down regulated and 28 miRNA was up regulated without significantly difference. So the miRNA in gill of M. chinensis might involve the environmental stress. 542 target genes were yielded when the 50 miRNA were hit to mRNA genome. And the target genes of differential expression miRNA were annotated by hitting to the NCBI database, and 4 genes hit to the COG, 1 genes hit to the GO, 5 genes hit to the KEGG and 11 genes hit to the nr database. The genes hit to the NCBI database include E3 ubiquitin-protein ligase, Wnt signaling pathway and Regulator of G-protein signaling 22.
2018-08-02 | GSE85561 | GEO
Project description:Atractylodes macrocephala is a traditional Chinese medicine in China. It is widely used in clinic and the market demand is great. The quality difference between A. chinensis(Bunge) Koidz and A. japonica Koidz.ex Kitam is great, which seriously affects its clinical curative effect. Determination of Atractylosin by High-performance liquid chromatography. Transcriptomics coupled with Metabonomics for elucidating differences between the components in A. chinensis(Bunge) Koidz and A. japonica Koidz.ex Kitam. It was found that ACS, ACC, PAL and NOS were the key genes and metabolites related to the synthesis of atractylodes macrocephala. The decrease of salicylic acid content in A. chinensis(Bunge) Koidz may lead to the decrease of its ability to mediate the elicitor of endophytic fungi, resulting in the down-regulation of the expression of TGAL4 transcription factor and the up-regulation of DOGL3 transcription factor in A. chinensis(Bunge) Koidz cells, which may affect the accumulation of Atractylosin. The study above provides a theoretical basis for elucidating the biosynthesis pathway of atractylosin in A. chinensis(Bunge) Koidz.
2024-09-28 | GSE278290 | GEO
Project description:The nematode Caenorhabditis elegans was treated with extracts of the Traditional Chinese Medicine plants Cuscuta chinensis and Eucommia ulmoides from the L4 stage. In aged worms (on the 7th and 12th day of adulthood) different health parameters were determined. Besides the prolongation of lifespan, we found that C. chinensis improved the swimming behavior, pharyngeal pumping rate, stress resistance, mechanosensation and memory of aged C. elegans. Furthermore, the extract treatment reduced the autofluorescence, which is a known biomarker of ageing. Thus, we concluded that C. chinensis is an overall healthspan enhancer. In contrast, the E. ulmoides extract specifically enhanced the lifespan and stress resistance of aged C. elegans, but did not improve any other health aspect. To reveal the mechanism behind the healthspan enhancing effects of C. chinensis, the transcriptome of treated and untreated C. elegans on the 12th day of adulthood was analysed. For comparison purposes, E. ulmoides treated nematodes were included in the analysis.
2024-10-30 | GSE274478 | GEO