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Lactobacillus

ABSTRACT: Lactobacillus salivarius and Lactobacillus reuteri

PROVIDER: PRJNA601071 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10901747.fastq.gz	Fastqsanger.gz
	SRR10901564.fastq.gz	Fastqsanger.gz
	SRR10901565.fastq.gz	Fastqsanger.gz

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Transcription profiling of Lactobacillus salivarius bile response_LS201

Project description:Porcine bile induced transcriptome of Lactobacillus salivarius

2009-07-16 | E-MEXP-2224 | biostudies-arrayexpress

The impact of glycerol on the metabolism of Lactobacillus reuteri

Project description:This SuperSeries is composed of the following subset Series: GSE11860: The impact of glycerol on the metabolism of Lactobacillus reuteri - Exploratory experiment GSE11861: The impact of glycerol on the metabolism of Lactobacillus reuteri - Main experiment Refer to individual Series

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Transcription profiling of Lactobacillus salivarius bile response_LS201bsh knockout

Project description:bile induced transcriptome of Lactobacillus salivarius LS201bsh ko.

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RNA sequencing analysis of transcriptomics to study the growth of Lactobacillus salivarius Ren in raffinose

Project description:Purpose: To understand the metabolic mechanism of Lactobacillus salivarius Ren in raffinose Methods: Samples of Lactobacillus salivarius Ren grown in glucose and raffinose were sequenced on the Illumina Hiseq platform. Three independent biological replicates were generated, including a total of six samples. Results: Raw data were firstly processed through in-house perl scripts to generate clean data, and then clean date were mapped to the reference genome, getting about 8-10 million total mapped reads per sample.

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Lactobacillus Membrane Vesicles

Project description:Bacterial membrane vesicles have been implicated in a broad range of functions in microbial communities from pathogenesis to gene transfer. Though first thought to be a phenomenon associated with Gram-negative bacteria, vesicle production in Staphylococcus aureus, Lactobacillus plantarum, and other Gram-positives has recently been described. Here we characterize MVs from three different Lactobacillus species (L. acidophilus, L. casei, and L. reuteri), determining that the size and protein composition of Lactobacillus-derived MVs have both similarities and differences with those produced by Gram-negative bacteria. Using proteomics, we identified more than 80 protein components from Lactobacillus-derived MVs, including some that were enriched in the vesicles themselves. For each species, vesicular proteins were categorized based on biological pathway and examined for subcellular localization signals in an effort to identify possible sorting mechanisms for MV proteins. Additionally, differences between MVs of other Lactobacillus species and Gram positive bacteria were highlighted. Information in this study will assist in elucidation of the formation and functions of MVs, as well as the development of therapeutic tools for vaccines, diagnosis, and therapeutic delivery.

2019-11-12 | PXD011278 | Pride

Inactivation of the Lactobacillus reuteri 100-23 ureC gene affects in vitro acid tolerance, and impairs ecological fitness in vivo

Project description:Lactobacillus reuteri 100-23 is an autochthonous inhabitant of the rodent gastrointestinal system that adheres to the non-secretory epithelium of the forestomach and forms biofilms. Microarray analysis of the expression profile of L. reuteri 100-23 cells harvested from the stomach of ex-Lactobacillus-free mice, compared to those of L. reuteri 100-23 in laboratory culture, revealed an in vivo upregulation of a urease gene cluster by greater than 50-fold. Genes for urease production were absent in all publically available Lactobacillus genome sequences except L. reuteri 100-23 and have recently been identified as specific to rodent strains of L. reuteri (Frese et al. 2011). In the current study, the urease enzyme was shown to be functional. Supplementation with 2% urea allowed L. reuteri 100-23 to increase the pH of the culture medium. A mutant strain of L. reuteri 100-23 was developed by insertional inactivation of the ureC gene, which encodes the largest subunit of the urease enzyme. The mutant strain was unable to hydrolyze urea to increase the pH of culture medium, and did not survive acid stress at pH 2.5 for 6 h, even in the presence of urea. In contrast, the wild type strain was still viable after 6 h when 2% urea supplementation was included. When mice free of lactobacilli were inoculated with a mixture of equal numbers of wild type L. reuteri 100-23 and ureC mutant cells, the wild type constituted 99% of the resulting Lactobacillus population in the stomach, caecum and jejunum after one week (108 cells/gram of sample). This study has therefore shown the importance of a functional urease enzyme in the ecological fitness of L. reuteri 100-23.

2012-09-30 | GSE36286 | GEO

Comparison of gene transcription in Caco-2 cells stimulated with Lactobacillus salivarius UCC118 with that in unstimulated cells.

Project description:Effect of Lactobacillus salivarius on gene transcription in caco-2 epithelial cells.

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Lactobacillus reuteri ATCC 55730: Before vs after exposure to 0.5% bile.

Project description:Transcriptional profiling of Lactobacillus reuteri ATCC 55730 mid-log cultures before vs after exposure to 0.5% bovine bile (oxgall). Two sets of array experiments were performed. One set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had been exposed to 0.5% bile for 15 minutes (bile shock). The other set compared the expression profiles of L. reuteri ATCC 55730 cells before bile exposure vs cells that had begun growing again in the presence of 0.5% bile (bile adaptation). Keywords: Stress response

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Inactivation of the Lactobacillus reuteri 100-23 ureC gene affects in vitro acid tolerance, and impairs ecological fitness in vivo

2012-09-30 | E-GEOD-36286 | biostudies-arrayexpress

Expression of RAW264.7 cells stimulated with RANKL and treated with supernatant from Lactobacillus reuteri

Project description:Analysis of gene expression in RAW264.7 cells stimulated for osteoclastogenesis and then treated with cell culture supernatant from Lactobacillus reuteri. Results will offer insight into targeted mechanisms suppressing osteoclastogenesis

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