Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: knock down of NRF2 in melanoma

PROVIDER: PRJNA601317 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10897128.fastq.gz	Fastqsanger.gz
	SRR10897129.fastq.gz	Fastqsanger.gz
	SRR10897130.fastq.gz	Fastqsanger.gz
	SRR10897131.fastq.gz	Fastqsanger.gz
	SRR10897132.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Homo sapiens

Project description:knock down of NRF2 in melanoma

| PRJNA600181 | ENA

Homo sapiens

Project description:NFATC2 knock-down on melanoma cell line A375

| PRJNA388509 | ENA

Homo sapiens

Project description:TYRP1 knock-down in human melanoma cell line (501Mel)

| PRJNA289013 | ENA

NFATC2 knock-down on melanoma cell line A375

Project description:RNA-seq on NFATC2 knock-down (siRNA) on melanoma cell line A375

2017-09-29 | GSE99466 | GEO

The sMAF transcription factor MAFG is a potent driver of melanoma

Project description:While common mutations in potent proto-oncogenes and tumor suppressors induce melanoma formation, no recurrent mutations associated with the late stages of melanomagenesis have been identified. Instead, non-mutational mechanisms such as the deregulation of transcriptiona or epigenetic programs typically promote the malignant progression and metastasis of melanoma. We have previously identified the small MAFG transcription factor MAFG as a critical target of the melanoma suppressor miRNA miRN-29, prompting an in-depth evaluation of the role of MAFG in melanoma. MAFG expression is elevated in melanoma compared to melanocytes and increases with tumor stage. Ectopic expression of MAFG in human melanocytes and melanoma cells enhances proliferation in virto and promotes melanoma growth in vivo. Moreover, MAFG overexpression in BRAFV600E; PTEN+/- mice accelerated melanomagenesis, significantly shortening overall survival. Despite being a critical NRF2 dimerization partner, NRF2 was dispensable for the effects of MAFG. Moreover, MAFG overexpression in melanoma had no effect on the activity of NRF2 transcriptional reporters, nor did it induce transcriptional programs associated with NRF2. RNA sequencing and pathway analysis revealed MAFG-mediated effects on melanoma-associated processes such as pigmentation. Indeed, MAFG downregulated MITF and its target genes in the pigmentation pathway in melanoma cells. We next analyzed the connection between MAFG and MITF and found that MAFG binds to MITF to impede its binding to melanocytic differentiation genes, thus promoting a less differentiated and more aggressive phenotype. Our study establishes MAFG as a potent driver of melanoma development through the relocation of MITF.

2024-10-09 | GSE274810 | GEO

Analysis of N1-methyladenosine methylome (m1A) enrichment level in ocular melanoma.

Project description:To investigate the function of N1-methyladenosine methylome (m1A) in ocular melanoma, we analyzed m1A enrichment level in ocular melanoma and melanocyte cell lines and established ALKBH3 knock down cell lines in 92.1.

2022-09-22 | GSE213748 | GEO

Transcriptomics profiling of the effect of inducible knock-down of the melanocyte inducing transcription factor (MITF) in melanoma cell lines

Project description:MITF plays critical role in development and differentiation of melanocytes and in the context of melanoma, as a lineage survival oncogene. Given its crucial role in melanoma biology, it is very difficult to generate complete knock-out (KO) of MITF and in our hands, those that were generated appear to behave differently than the effect observed using siRNA mediated knock-down, possibly indicative of selection. In order to overcome the limitation of the transient effect of siRNA and study the effect of MITF depletion over a longer period of time, we carried out transcriptomic analyses of Doxycycline inducible shMITF knock down after 8 days in 2 melanoma cell lines MeWo (CVCL_0445) and SkMel 28 (CVCL_0526)

2024-11-08 | GSE255256 | GEO

polyA+ and total RNA sequencing profiling of UNR (CSDE1) knock down

Project description:Investigation of the effects of UNR (CSDE1) knock-down on transcript levels of Melanoma Cells.

2016-10-31 | E-MTAB-3805 | biostudies-arrayexpress

Ribosome profiling of UNR (CSDE1) KD

Project description:Investigation of the effects of UNR (CSDE1) knock-down on translation in Melanoma Cells using Ribosome Profiling

2016-11-07 | E-MTAB-3815 | biostudies-arrayexpress

Project description:TAL1 knock down

| PRJNA125301 | ENA