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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

ABSTRACT: The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

PROVIDER: PRJNA601786 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10905200.fastq.gz	Fastqsanger.gz
	SRR10905201.fastq.gz	Fastqsanger.gz
	SRR10905202.fastq.gz	Fastqsanger.gz
	SRR10905203.fastq.gz	Fastqsanger.gz
	SRR10905204.fastq.gz	Fastqsanger.gz

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [RNA-seq]

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Project description:Mediator is a multi-protein complex which facilitates the initial steps of gene transcription. Mediator 12 (MED12) and MED13 are components of a module that reversibly associates with the core Mediator complex, and both positively and negatively regulates gene expression. Here, in an Arabidopsis mutant screen for factors required for morc1 de-repressed gene expression, we identified MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes lacking active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discovered that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that the light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes showed little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from non-active to active states.

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-04-29 | GSE143835 | GEO