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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE143835 | GEO | 2020/04/29

REPOSITORIES: GEO

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Mediator12 and 13 act as conditional anti-silencing factors in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-12-31 | GSE123563 | GEO

The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [RNA-seq]

Project description:Mediator is a multi-protein complex which facilitates the initial steps of gene transcription. Mediator 12 (MED12) and MED13 are components of a module that reversibly associates with the core Mediator complex, and both positively and negatively regulates gene expression. Here, in an Arabidopsis mutant screen for factors required for morc1 de-repressed gene expression, we identified MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes lacking active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discovered that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that the light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes showed little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from non-active to active states.

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [ChIP-seq]

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The characterization of Mediator 12 and 13 as conditional positive gene regulators in Arabidopsis.

Project description:Mediator 12 (MED12) and MED13 are components of the Mediator multi-protein complex, that facilitates the initial steps of gene transcription. Here, in an Arabidopsis mutant screen, we identify MED12 and MED13 as positive gene regulators, both of which contribute broadly to morc1 de-repressed gene expression. Both MED12 and MED13 are preferentially required for the expression of genes depleted in active chromatin marks, a chromatin signature shared with morc1 re-activated loci. We further discover that MED12 tends to interact with genes that are responsive to environmental stimuli, including light and radiation. We demonstrate that light-induced transient gene expression depends on MED12, and is accompanied by a concomitant increase in MED12 enrichment during induction. In contrast, the steady-state expression level of these genes show little dependence on MED12, suggesting that MED12 is primarily required to aid the expression of genes in transition from less-active to more active states.

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

Project description:The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [ChIP-seq]

Project description:The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [ChIP-seq]

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The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [RNA-seq]

Project description:The characterization of Mediator12 and 13 as conditional positive gene regulators in Arabidopsis [RNA-seq]

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Mediator12 and 13 act as conditional anti-silencing factors in Arabidopsis [ChIP-seq]

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