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Single cell RNA sequencing of adult regenerating mouse digit tips

ABSTRACT: Single cell RNA sequencing of adult regenerating mouse digit tips

PROVIDER: PRJNA601983 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10909674.fastq.gz	Fastqsanger.gz
	SRR10909675.fastq.gz	Fastqsanger.gz
	SRR10909676.fastq.gz	Fastqsanger.gz
	SRR10909677.fastq.gz	Fastqsanger.gz
	SRR10909678.fastq.gz	Fastqsanger.gz

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Single cell RNA sequencing of adult regenerating mouse digit tips

Project description:Single cell RNA sequencing of adult regenerating mouse digit tips

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Single cell RNA sequencing of adult regenerating mouse digit tips

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Single cell RNA sequencing of adult regenerating mouse digit tips

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Project description:Here, we investigate the origin and nature of blastema cells that regenerate the adult murine digit tip. We show that Pdgfra-expressing mesenchymal cells in uninjured digits establish the regenerative blastema and are essential for regeneration. Single cell profiling shows that the mesenchymal blastema cells are distinct from both uninjured digit and embryonic limb/digit Pdgfra-positive cells. This unique blastema state is environmentally determined; dermal fibroblasts transplanted into the regenerative, but not non-regenerative, digit express blastema markers and contribute to bone regeneration. Moreover, lineage tracing with single cell profiling indicates that endogenous osteoblasts/osteocytes acquire a blastema mesenchymal transcriptional state and contribute to both dermis and bone regeneration. Thus, mammalian digit tip regeneration occurs via a distinct adult mechanism where the regenerative environment promotes acquisition of a unique blastema state that allows cells from tissues like bone to contribute to regeneration of other mesenchymal tissues such as the dermis.

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