Project description:The parasite species complex Anisakis simplex sensu lato (Anisakis simplex sensu stricto; (A. simplex s.s.), A. pegreffii, A. simplex C) is the main cause of severe anisakiasis (allergy) worldwide and is now an important health matter. In this study, the relationship of this Anisakis species complex and their allergenic capacities is assessed by studying the differences between the two most frequent species (A. simplex s.s., A. pegreffii) and their hybrid haplotype by studying active L3 larvae parasiting Merluccius merluccius.
Project description:The total proteomes of Anisakis simplex s.s., A. pegreffii and their hybrid genotype have been compared by quantitative proteomics (iTRAQ approach), which considers the level of expressed proteins. A total of 1,976 proteins have been identified using public databases. One hundred ninety six proteins were found significantly differentially expressed; results of pairwise Log2 ratio comparisons among them were statistically treated and supported in order to convert them into discrete character states. This comparison selected thirty six proteins as discriminant biomarkers among A. simplex, A. pegreffii and their hybrid genotype; eighteen of these biomarkers, encoded by nine loci, are specific allergens of Anisakis (Ani s7, Ani s8, Ani s12 and Ani s14) and other (Ancylostoma secreted) is a common nematodes venom allergen.
2020-08-17 | PXD019289 | Pride
Project description:De novo transcriptome of Anisakis pegreffi at third larval stage
Project description:DNA methylation is a pivotal process that regulates gene expression and facilitates rapid adaptation to challenging environments. The pine wood nematode (PWN; Bursaphelenchus xylophilus), the causative agent of pine wilt disease, survives and spreads at low temperatures through third-stage dispersal larvae, making it a major pathogen for pine wood in Asia. To comprehend the impact of DNA methylation on the formation and environmental adaptation of third-stage dispersal larvae, we conducted whole-genome bisulfite sequencing and transcriptional sequencing on both the third-stage dispersal larvae and three other stages propagative larvae of PWN.