Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Knockout of CNOT1 and CNOT10 in primary CD4+ T-cells causes a type-1 interferon response

ABSTRACT: Knockout of CNOT1 and CNOT10 in primary CD4+ T-cells causes a type-1 interferon response

PROVIDER: PRJNA602875 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10957159.fastq.gz	Fastqsanger.gz
	SRR10957160.fastq.gz	Fastqsanger.gz
	SRR10957161.fastq.gz	Fastqsanger.gz
	SRR10957162.fastq.gz	Fastqsanger.gz
	SRR10957163.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Knockout of CNOT1 and CNOT10 in primary CD4+ T-cells causes a type-1 interferon response

Project description:We report a type 1 interferon response accompanying polyclonal knockout of CNOT1 or CNOT10 in primary human CD4+ T-cells harvested from healthy donors

2020-01-24 | GSE144142 | GEO

Ribosome Profiling with CNOT1 depletion

Project description:Ribosome profiling in HEK293 cells with and without the depletion of CNOT1. This allows the determination of translational efficiency and nucleotide resolution of ribosome occupancy.

2021-08-23 | GSE158141 | GEO

ChIP-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice

Project description:Gene expression is balanced by transcription and mRNA degradation. Shortening of polyadenosine (poly(A)) tails (deadenylation) is the initial step in the decay of most mRNAs. However, the mechanism by which deadenylation controls mRNA expression is not fully understood. This experiment aimed to determine changes in transcriptional activity in livers of control and Cnot1 tamoxifen-inducible liver-specific knockout mice. Control and Cnot1 tamoxifen-inducible liver-specific knockout mice placed on tamoxifen-containing food at 6 weeks of age for 2 weeks. Lysates from livers were subjected to ChIP assay with Histone H3 (tri methyl K4) (H3K4me3) antibody (ab8580; abcam) using SimpleChIP Enzyatic Chromatin IP Kit (#9003; Cell Signaling Technology). Libraries for DNA sequence were prepared from DNA isolated by ChIP assays with a KAPA Hyper Prep Kit (Illumina). 109 base-pair pair-end read DNA-seq was performed with Hiseq PE Rapid Cluster Kit v2-HS and Hiseq Rapid SBS Kit v2-HS (200 Cycle) on Illumina Hiseq2500.

2020-03-30 | E-MTAB-5887 | biostudies-arrayexpress

RNA-seq of livers in control and Cnot1 tamoxifen-inducible liver-specific knockout mice

Project description:Gene expression is balanced by transcription and mRNA degradation. Shortening of polyadenosine (poly(A)) tails (deadenylation) is the initial step in the decay of most mRNAs. However, the mechanism by which deadenylation controls mRNA expression is not fully understood. This experiment aimed to determine changes in gene expression and mRNA stability in livers of control and Cnot1 tamoxifen-inducible liver-specific knockout mice. For comprehensive mRNA half-life profiling, we injected control and Cnot1 tamoxifen-inducible liver-specific knockout mice, which were placed on a tamoxifen-containing diet at 6 weeks of age for 2 weeks, intraperitoneally with 2 mg actinomycin D per g body weight for 4 and 8 hr. 1 g of total RNA was used for RNA-seq library preparation with Illumina TruSeq Stranded mRNA LT Sample Prep Kit. 109 base-pair pair-end read RNA-seq was performed with Hiseq PE Rapid Cluster Kit v2-HS and Hiseq Rapid SBS Kit v2-HS (200 Cycle) on Illumina Hiseq2500.

2020-03-30 | E-MTAB-5901 | biostudies-arrayexpress

Ribosome Profiling with CNOT1 depletion

Project description:Ribosome Profiling with CNOT1 depletion

| PRJNA664116 | ENA

Interleukin-10 causes interferon γ-dependent emergency myelopoiesis

Project description:Interleukin-10 causes interferon γ-dependent emergency myelopoiesis

| PRJNA721937 | ENA

Interleukin-10 causes interferon-γ-dependent emergency myelopoiesis

Project description:Interleukin-10 causes interferon-γ-dependent emergency myelopoiesis

| PRJNA613303 | ENA

mRNA stability experiments with control or CNOT1-targeting siRNA treatment in HEK293 cells

Project description:Determination of mRNA half-life in HEK293 cells with and without the depletion of CNOT1.

2021-08-22 | GSE158619 | GEO

CNOT1 and Transcriptomic Landscape of a HeLa Cell Line

Project description:Purpose: The goals of this study are to compare NGS-derived total transcriptome profiling (RNA-seq) of CNOT1-depleted cells (using siRNA) with control HeLa cells before and after DNA damage to examine quantitative gene expression.

2019-12-05 | GSE141496 | GEO

RNA-seq of ER-targeted and cytosolic mRNAs with control or CNOT1-targeting siRNA treatment in HEK293 cells

Project description:Determination of mRNA localisation in HEK293 cells with and without the depletion of CNOT1

2021-09-07 | GSE183148 | GEO