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RNA-seq of murine breast cancer cell line AT3 infected with F. nucleatum

ABSTRACT: RNA-seq of murine breast cancer cell line AT3 infected with F. nucleatum

PROVIDER: PRJNA602883 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10957259.fastq.gz	Fastqsanger.gz
	SRR10957260.fastq.gz	Fastqsanger.gz
	SRR10957261.fastq.gz	Fastqsanger.gz
	SRR10957262.fastq.gz	Fastqsanger.gz
	SRR10957263.fastq.gz	Fastqsanger.gz

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RNA-seq of murine breast cancer cell line AT3 infected with F. nucleatum

Project description:Fusobacterium nucleatum (F. nucleatum) is implicated to exacerbate colorectal cancer. However, there is also evidence that the bacterium accumulates on other cancer types such as breast cancer. In order to better understand the transcriptional response of breast cancer cells to infection with F. nucleatum, we performed RNA-seq of the murine breast cancer cell AT3 with F. nucleatum.

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Expression data from colorectal cancer and the adjacent normal tissues infected without F. nucleatum

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Project description:Expression data of miRNA profile from colorectal cancer and the adjacent normal tissues infected with F. nucleatum. Colorectal cancer(CRC) is among the most common cancer types in the world and one of the most lethal cancers. Fusobacterium nucleatum (F. nucleatum) plays an etiologic role in the development of colorectal cancer, but specific tumor molecules involved in the progression of CRC induced by F. nucleatum is not clear. This study investigated some miRNAs and the genes involved in the progression of F. nucleatum-induced colorectal cancer by Affymetrix miRNA microarray technology. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated and down-regulated genes between Colorectal cancer and the adjacent normal tissues (CONTROL) infected without F. nucleatum . The miRNA array was performed using the affymetrix GeneChip® miRNA 3.0 Array (Affymetrix, Santa Clara, California, United States). The chip was processed using a commercial Affymetrix array service (GeneTech Biotechnology Limited Company, Shanghai, China). The affymetrix GeneChip® miRNA 3.0 Array contains 2,999 probe sets unique to human, mouse and rat pre-miRNA hairpin sequences, 2,216 human snoRNA and scaRNA probe sets and covers 153 organisms (19,724 probe sets). Raw data sets were extracted from all Cel files (raw intensity file) after scanning of slides. These raw data sets were separately analyzed using Expression Console and GeneSpring GX12.5 software followed by differential miRNA expression, fold change & cluster analysis.

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