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The G-protein coupled receptor GPR8 regulates secondary metabolism and mating associated chemical communication in Trichoderma reesei

ABSTRACT: The G-protein coupled receptor GPR8 regulates secondary metabolism and mating associated chemical communication in Trichoderma reesei

PROVIDER: PRJNA603960 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10989748.fastq.gz	Fastqsanger.gz
	SRR10989749.fastq.gz	Fastqsanger.gz
	SRR10989750.fastq.gz	Fastqsanger.gz
	SRR10989751.fastq.gz	Fastqsanger.gz
	SRR10989752.fastq.gz	Fastqsanger.gz

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The G-protein coupled receptor GPR8 regulates secondary metabolism and mating associated chemical communication in Trichoderma reesei

Project description:GPR8 exerts a light dependent function on secondary metabolism, which is in part mediated by YPR2 and the function of SOR7

2020-01-31 | GSE144529 | GEO

A G-protein coupled receptor supports methionine response in Trichoderma reesei

Project description:We investigated the function of the G-protein coupled receptor 72004 in Trichoderma reesei and found that it is involved in methionine response and gene expression in light and darkness

2024-01-01 | GSE222724 | GEO

A G-protein coupled receptor supports methionine response in Trichoderma reesei

Project description:A G-protein coupled receptor supports methionine response in Trichoderma reesei

| PRJNA923275 | ENA

Transcriptome analysis of T. reesei CBS999.97, backcrossed female fertile strains in QM6a genetic background and QM6a upon mating

Project description:The aim of this study was to evaluate changes in the transcriptome in the mentioned strains during mating conditions

2017-11-07 | GSE89104 | GEO

The role of pheromone receptors for communication and mating in Hypocrea jecorina (Trichoderma reesei).

Project description:Discovery of sexual development in the ascomycete Trichoderma reesei (Hypocrea jecorina) as well as detection of a novel class of peptide pheromone precursors in this fungus indicates promising insights into its physiology and lifestyle. Here we investigated the role of the two pheromone receptors HPR1 and HPR2 in the H. jecorina pheromone-system. We found that these pheromone receptors show an unexpectedly high genetic variability among H. jecorina strains. HPR1 and HPR2 confer female fertility in their cognate mating types (MAT1-1 or MAT1-2, respectively) and mediate induction of fruiting body development. One compatible pheromone precursor-pheromone receptor pair (hpr1-hpp1 or hpr2-ppg1) in mating partners was sufficient for sexual development. Additionally, pheromone receptors were essential for ascospore development, hence indicating their involvement in post-fertilisation events. Neither pheromone precursor genes nor pheromone receptor genes of H. jecorina were transcribed in a strictly mating type dependent manner, but showed enhanced expression levels in the cognate mating type. In the presence of a mating partner under conditions favoring sexual development, transcript levels of pheromone precursors were significantly increased, while those of pheromone receptor genes do not show this trend. In the female sterile T. reesei strain QM6a, transcriptional responses of pheromone precursor and pheromone receptor genes to a mating partner were clearly altered compared to the female fertile wild-type strain CBS999.97. Consequently, a delayed and inappropriate response to the mating partner may be one aspect causing female sterility in QM6a.

| S-EPMC3462998 | biostudies-literature

Trichoderma reesei QM6a CGH self hybridization

Project description:We perform a self hybridisation comprative genomic hybridization (CGH) in order to validate the probe tiling design we done on Trichoderma reesei. This hybridization was done using QM6a wild type strain.

2010-03-16 | GSE17752 | GEO

Identification of microRNA-like RNAs in the filamentous fungus Trichoderma reesei by Solexa sequencing

Project description:MicroRNAs (miRNAs) are small non-coding RNAs capable of negatively regulating gene expression. Trichoderma reesei is an industrial filamentous fungus that can secrete abundant hydrolases for cellulosic biofuels. Recently, microRNA-like RNAs (milRNAs) were discovered in several filamentous fungi rather than T. reesei. The purpose of this study was to explore the presence of milRNA in T. reesei, to characterize the differential expression of T. reesei milRNA under cellulose induction, and to reveal the target genes of milRNA involved in cellulase production. Two small RNA libraries of cellulose induction (IN) or non-induction (CON) were generated and sequenced using Solexa sequencing technology. A total of 664,463 and 529,545 unique sequences, representing 1,271 and 1,021 unique small RNAs, were obtained from the IN and CON samples, respectively. Thirteen milRNAs were finally identified in T. reesei using the hairpin structure analysis. The milRNAs profiles obtained in deep sequencing were validated by RT-qPCR assay. The miRanda program predicts a number of potential targets for T. reesei milRNAs, including several hydrolases and carbon catabolite repressor Cre1.The presence and differential expression of T. reesei milRNAs, along with their predicted targets indicate that milRNAs might play a regulatory role in cellulase induction. This work lays foundation for further functional study of fungal milRNAs and their industrial application.

2014-12-31 | GSE46679 | GEO

Trichoderma reesei

Project description:Transcriptome analysis of T. reesei CBS999.97, backcrossed female fertile strains in QM6a genetic background and QM6a upon mating

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Project description:To investigate the potential role in mycoparasitism, microarrays were used to examine T. reesei transcript levels when confronted with a potential prey (the plant pathogen Rhizoctonia solani) before contact, during first physical contact and during overgrowth of the host.

2011-07-01 | GSE23410 | GEO

Chemical communication in Capnodis tenebrionis beetles

Project description:Chemical communication strategies of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae): ecological implications for intraspecific and host interactions

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