Project description:Global transcriptional analysis of human extended pluripotent stem cells, human primed pluripotent stem cells, mouse extended pluripotent stem cells, and mouse embryonic stem cells by RNA-seq

| PRJNA319819 | ENA

Programming human pluripotent stem cells into adipocytes

Project description:Programming human pluripotent stem cells into adipocytes

| PRJNA144087 | ENA

Metabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors

Project description:Metabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.

2021-06-29 | ST002055 | MetabolomicsWorkbench

Metabolites of human neurons and stem cells (siNeuron metabolomics)

Project description:There are a total of four samples each for this analysis. Each sample consists of the cells grown on three 10 cm culture plates. Each plate should have 2x106 cells for a total of 6x106 cells per sample when all three plates are combined. The first sample is undifferentiated human embryonic stem cells, the second sample is human glutamatergic neurons derived from those human embryonic stem cells, the third sample is undifferentiated human induced pluripotent stem cells and the fourth sample is human glutamatergic neurons derived from those human induced pluripotent stem cells.

2017-06-19 | ST000768 | MetabolomicsWorkbench

Derivation of airway basal stem cells from human pluripotent stem cells

Project description:Derivation of airway basal stem cells from human pluripotent stem cells

| PRJNA596381 | ENA

Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

Project description:Two distinct trophectoderm lineage stem cells from human pluripotent stem cells

| PRJNA565033 | ENA