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Derivation of trophoblast stem cells from naïve human pluripotent stem cells

ABSTRACT: Derivation of trophoblast stem cells from naïve human pluripotent stem cells

PROVIDER: PRJNA577070 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10257227.fastq.gz	Fastqsanger.gz
	SRR10267027_1.fastq.gz	Fastqsanger.gz
	SRR10267027_2.fastq.gz	Fastqsanger.gz
	SRR10257228.fastq.gz	Fastqsanger.gz
	SRR10267028_1.fastq.gz	Fastqsanger.gz

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Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Naïve human pluripotent stem cells (hPSCs) provide a unique experimental platform of cell fate decisions during pre-implantation development, but their lineage potential remains incompletely characterized. As naïve hPSCs share transcriptional and epigenomic signatures with trophoblast cells, it has been proposed that the naïve state may have enhanced predisposition for differentiation along this extraembryonic lineage. Here we examined the trophoblast potential of isogenic naïve and primed hPSCs. We found that naïve hPSCs can directly give rise to human trophoblast stem cells (hTSCs) and undergo further differentiation into both extravillous and syncytiotrophoblast. In contrast, primed hPSCs do not support hTSC derivation, but give rise to non-self-renewing cytotrophoblasts in response to BMP4. Global transcriptome and chromatin accessibility analyses indicate that hTSCs derived from naïve hPSCs acquire features of pre-implantation trophectoderm. The derivation of hTSCs from naïve hPSCs will enable elucidation of early mechanisms that govern normal human trophoblast development and associated pathologies.

2020-02-13 | GSE138761 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

2020-02-13 | GSE138688 | GEO

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells [ATAC-seq]

| PRJNA577096 | ENA

Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

Project description:Derivation of trophoblast stem cells from naïve human pluripotent stem cells [RNA-seq]

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells

| PRJNA757782 | ENA

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

| PRJNA757786 | ENA

Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq)

Project description:Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq)

| PRJNA666919 | ENA

Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

Project description:Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [bulk RNA-seq]

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Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq]

Project description:Trophoblast stem cells (TSC) have recently been derived from human embryos, and early first trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a BMP4-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSC) into functional trophoblast; however, those trophoblast could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to a newly-developed TSC media, to derive bona-fide TSC. We show that these cells resemble placenta- and naïve hPSC-derived TSC, based on their transcriptome, as well as in vitro and in vivo differentiation potential. We conclude that primed hPSC can be used to generate functional TSC, through a simple protocol which can be applied to a widely-available set of existing hPSC, including induced pluripotent stem cells derived from patients with known birth outcomes.

2022-05-16 | GSE182790 | GEO

Derivation of trophoblast stem cells (TSTerm cells) from term placentas (RNA-seq)

Project description:Purpose: To understand the regulatiory mechanisms of trophoblast stemness and differentiation gene expression. Methods: Three trophoblast stem cell lines were derived from individual term placnta, TSTerm #1 and TSTerm #2 TSTerm #3. The three TSTerm cell lines were differentiated into syncytiotrophoblasts (STB：ST-TSTerm #1, ST-TSTerm #2, and ST-TSTerm #3) and extravillous trophoblasts (EVT：EVT-TSTerm #1, EVT-TSTerm #2 and EVT-TSTerm #3). TS#1GCM1-KO cell line was derived from TSTerm #1 cells by depletion of GCM1 gene. ST-TS#1GCM1-KO cells were differentiated from TS#1GCM1-KO cells. Additionally, CTBTerm #1, CTBTerm #2, and CTBTerm #3 were isolated from individual placentae. The mRNA profiles of tatal fourteen samples as mentioned above were generated by sequencing, using NovaSeq Sequencing System. The raw data were analyzed by CLC Genomics Workbench v10. Conclusion: We elucidated reciprocal regulation of trophoblast stemness and differentiation gene expression, which facilitated the derivation of TS cells from term placentas.

2021-12-21 | GSE158901 | GEO

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