Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: CRISPR-mediated biocontainment: Data for figure S2

PROVIDER: PRJNA604281 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10997710_1.fastq.gz	Fastqsanger.gz
	SRR10997710_2.fastq.gz	Fastqsanger.gz
	SRR10997711_1.fastq.gz	Fastqsanger.gz
	SRR10997711_2.fastq.gz	Fastqsanger.gz
	SRR10997712_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Project description:CRISPR-mediated biocontainment: Data for figure 3A

| PRJNA604277 | ENA

Project description:CRISPR-mediated biocontainment: Data for figure 1B

| PRJNA604267 | ENA

ChIP-seq of GFP-tagged dCoREST, dLSD1, dL(3)mbt and dG9a in D. melanogaster S2 cell lines

Project description:We employed CRISPR/Cas-mediated genome editing to generate S2 cell lines expressing GFP-tagged dCoREST, dL(3)mbt, dLSD1 and dG9a, respectively. This allowed us to determine the genome-wide binding profiles for these proteins by ChIP-seq using the same antibody (anti-GFP) in each case.

2019-11-04 | E-MTAB-8341 | biostudies-arrayexpress

Kservage_20241202_NCOMMS-24-75296_A repurposed AMP binding domain reveals mitochondrial protein AMPylation as a regulator of cellular metabolism

Project description:Raw files for proteomics datasets: Table S2 and Figure S1 = file# 3415 (AMP GlnA peptide) and file#s 3698 (DA), 3699 (WT), and 3700 (VA) Table S3 and Figure 5 = files #s 3670 (DA) and 3673 (UC) Figure 6 = file #s 4543 (DA) and 4544 (UC) Figure S11 = file# 4544 Table S1= file# 2980

2024-12-02 | MSV000096576 | MassIVE

Project description:the NGS data from CRISPR-mediated HDR

| PRJNA644281 | ENA

Project description:S2 Metagenome data

| PRJNA790701 | ENA

The Drosophila BEAF Insulator Protein Interacts with the Polybromo Subunit of the PBAP Chromatin Remodeling Complex [Mnase-seq]

Project description:We examine the effect of this interaction on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells. We examine the effect of the interaction between BEAF and polybromo on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells.

2022-03-05 | GSE197574 | GEO

Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers

Project description:Using CRISPR-Cas9 to tag endogenous remodeler subunits in Drosophila melanogaster S2 cells, we demonstrate that developmental gene transcription requires SWI/SNF-type complexes, primarily to maintain distal enhancer accessibility.

2023-10-24 | PXD036007 | Pride

Quantitative Proteomic Atlas Uncovering the widespread Ubiquitylation regulation in the Embryo of Germinating Rice Seed

Project description:Using high affinity K-Ɛ-GG antibody integrating highly sensitive MS, we investigated the quantitative atlas of ubiquitylome as well as the proteome in the embryos of germinated rice seed. A total of 7507 proteins were identified, and 5018 were precisely quantified, the mass errors and peptides length distribution showed the high mass accuracy, andthe PCA result displayed the well repeatability (Figure S3, Table S2). Of these quantified proteins, 764(65.3%) were found in the ubiquitylome, this high overlapping ascribed to the high resolution of MS.

2022-02-23 | PXD006526 | Pride

Hepatitis B virus pre-S2 mutant LHBS induced DNA breakages and genomic instability.

Project description:Although hepatitis B virus (HBV) has been established to cause hepatocellular carcinoma (HCC), the exact mechanism remains to be clarified. Type II ground glass hepatocytes (GGH) harboring the HBV pre-S2 mutant large surface protein have been recognized as morphologically distinct hallmark of HCC in advanced stages of chronic HBV infection. Considering its pre-neoplastic nature, we hypothesized that type II GGH may exhibit high genomic instability, which is important for the carcinogenic process in chronic HBV carriers. In this study we found that pre-S2 mutant LHBS directly interacted with importin 1, the key factor that recognizes cargos undergoing nuclear transportation mediated by the importin 1 associated nuclear pore complex (NPC). By interacting with importin 1, which inhibits its function as an NPC factor, pre-S2 mutant LHBS blocked nuclear transportation of an essential DNA repair and recombination factor Nijmegen breakage syndrome 1 (NBS1) upon DNA damage, thereby delaying formation of nuclear foci at the sites of DNA double strand breaks. Pre-S2 mutant LHBS was also found to block NBS1-mediated homologous recombination repair and induce multi-nucleation of cells. In addition, pre-S2 mutant LHBS transgenic mice showed genomic instability indicated by increased global gene copy number variations (CNV), which were significantly higher than those in hepatitis B virus X mice, indicating that pre-S2 mutant LHBS is the major viral oncoprotein inducing genomic instability in HBV-infected hepatocytes. Consistently, the human type II GGHs in HCC patients exhibited increased DNA double-strand breaks representing significant genomic instability. In conclusion, type II GGH harboring HBV pre-S2 mutant oncoprotein represents a high-risk marker for the loss of genome integrity in chronic HBV carriers and explains the complex chromosome changes in HCCs.

2014-09-13 | GSE61378 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data