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Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers

ABSTRACT: Using CRISPR-Cas9 to tag endogenous remodeler subunits in Drosophila melanogaster S2 cells, we demonstrate that developmental gene transcription requires SWI/SNF-type complexes, primarily to maintain distal enhancer accessibility.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Cell Culture

SUBMITTER: Richard Imre

LAB HEAD: Alexander Stark

PROVIDER: PXD036007 | Pride | 2023-10-24

REPOSITORIES: Pride

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			Action	DRS
	20200729_QExHFX2_RSLC8_Serebreni_Stark_IMP_onbead_try_dig_40per_4x3samples_Flybase_step2_Limma.pdResult	Other
	20200729_QExHFX2_RSLC8_Serebreni_Stark_IMP_onbead_try_dig_40per_cont_parternal_rep1_10.raw	Raw
	20200729_QExHFX2_RSLC8_Serebreni_Stark_IMP_onbead_try_dig_40per_cont_parternal_rep2_11.raw	Raw
	20200729_QExHFX2_RSLC8_Serebreni_Stark_IMP_onbead_try_dig_40per_cont_parternal_rep3_12.raw	Raw
	20200804_QExHFX2_RSLC8_Serebreni_Stark_IMP_onbead_try_dig_40per_sample_Brd7_rep_1_7.raw	Raw

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Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers.

Hendy Oliver O Serebreni Leonid L Bergauer Katharina K Muerdter Felix F Huber Lukas L Nemčko Filip F Stark Alexander A

Molecular cell 20220915 19

Gene transcription is a highly regulated process in all animals. In Drosophila, two major transcriptional programs, housekeeping and developmental, have promoters with distinct regulatory compatibilities and nucleosome organization. However, it remains unclear how the differences in chromatin structure relate to the distinct regulatory properties and which chromatin remodelers are required for these programs. Using rapid degradation of core remodeler subunits in Drosophila melanogaster S2 cells, ...[more]

PMID: 36113480

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