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Effect of C3a treatment over cell death of pancreatic islets ex vivo

ABSTRACT: Effect of C3a treatment over cell death of pancreatic islets ex vivo

PROVIDER: PRJNA604618 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11007906_1.fastq.gz	Fastqsanger.gz
	SRR11007906_2.fastq.gz	Fastqsanger.gz
	SRR11007907_1.fastq.gz	Fastqsanger.gz
	SRR11007907_2.fastq.gz	Fastqsanger.gz
	SRR11007908_1.fastq.gz	Fastqsanger.gz

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Effect of C3a treatment over cell death of pancreatic islets ex vivo

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Differences in gene expression in islets isolated from pancreata donated after Cardiac Death relative to Brain Death

Project description:The decreasing numbers of Donation after Brain Death (DBD) donors necessitates the comprehensive evaluation of Donation after Cardiac Death Donors (DCD) as a source of pancreata. The aim of this study was to characterize pancreata and islets from DCD and DBD donors with respect to markers of cellular stress that may indicate compromised islet quality. Immunohistochemical staining of pre-isolation pancreas biopsies found increased numbers of caspase 3 positive islets in DBD, while markers of oxidative stress (nitrotyrosine, CML, and HNE) were elevated in DCD. Assessment of islet quality by standard (yield, morphology, fluorescence microscopy, and glucose stimulated insulin secretion) and novel methods (flow cytometry, HPLC quantification of ATP) did not reveal significant differences. However, the post culture loss of DCD islets was increased compared to DBD, and DCD islets showed delayed functional potency when transplanted into diabetic NOD.scid mice. Microarray analysis of cultured islets showed increased expression of multiple stress pathway related genes in DCD compared to DBD. Together these data indicate that the current standard donor management, pancreas recovery and preservation practices are insufficient to quench the oxidative stress injury suffered by DCD islets which leads to loss in culture and may complicate their use in clinical transplant. Keywords: cell type comparison

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Project description:Gene expression profiles comparing FCCP, CCCP, 6OH-BDE47, Ethylenglycol, TCP, PCP, Aniline, chloroaniline, DMSO in C3A cells

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Project description:Expression profiling using the UPenn Mouse PancChip 5.0 was used to elucidate the genetic mechanisms of PANDER-induced cell death in pancreatic islets. Murine islets were treated with PANDER (PANcreatic DERived factor) for 48 or 72 h (n=4 and n=3 respectively). Following linear amplification, the RNA was matched for purity using Quantitative PCR.

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