Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont.

2016-06-01 | GSE67894 | GEO

Tissue-Specific Transcriptomics and Proteomics of a Filarial Nematode and its Wolbachia Endosymbiont

Project description:We sequenced total RNA from Dirofilaria immitis in order to generate the first tissue-specific gene expression profile of a filarial nematode and its Wolbachia endosymbiont. Examination of transcript levels in 7 different Dirofilaria immitis tissues, in duplicate, using Illumina GAIIx.

2016-06-01 | E-GEOD-67894 | biostudies-arrayexpress

Tyrophagus putrescentiae isolate:UMMZ BMOC 17-0108-002 PMR

Project description:Tyrophagus putrescentiae and associated fungi and bacteria

| PRJNA598686 | ENA

Identification of cross-species microRNA-based Communication in Brugia malayi with its endosymbiont Wolbachia

Project description:Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. The largest identified cluster is downregulated in adult females and enriched in zinc finger domains, helicase domains, and DNA binding domains suggesting this microRNA cluster may have regulatory control over a large proportion of adult female specific mRNA genes. MicroRNA-like molecules are identified as produced by the Wolbachia endosymbiont, providing evidence for direct nucleic acid-based interdomain communication between filarial nematodes and their bacterial obligate endosymbiont.

2022-01-15 | GSE143730 | GEO

Solitalea symbiont of Tyrophagus putrescentiae strain:mix of clutures

Project description:Solitalea symbiont of Tyrophagus putrescentiae strain:mix of clutures Genome sequencing

| PRJNA1154563 | ENA

Allergens and Wolbachia proteins in Tyrophagus putrescentiae

Project description:Three populations of T. putrescentiae with a known microbiome were analyzed using label-free nanoLC-MS/MS analysis. Two homogenization procedures were performed - one with a glass homogenizer (for the 3X6 dataset) and the second with beads/shards (for the 3X3 dataset). The latter was used due to its potential higher yield of bacterial proteins. Two raw datasets (3X6 and 3X3) from the nanoLC-MS/MS analysis are included. Compressed (zipped) combined_txt folders of database searches in MaxQuant are included. In addition, used databases (*.fasta) are provided.

2021-08-24 | MSV000088016 | MassIVE

Wolbachia endosymbiont of Onchocerca lupi isolate:Wolbachia Endosymbiont of Onchocerca lupi

Project description:Wolbachia endosymbiont of Onchocerca lupi isolate:Wolbachia Endosymbiont of Onchocerca lupi Genome sequencing

| PRJNA933935 | ENA