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RNA-seq of GAP43 overexpressed and control SW620 cancer cells

ABSTRACT: RNA-seq of GAP43 overexpressed and control SW620 cancer cells

PROVIDER: PRJNA606268 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11074200_1.fastq.gz	Fastqsanger.gz
	SRR11074200_2.fastq.gz	Fastqsanger.gz
	SRR11074201_1.fastq.gz	Fastqsanger.gz
	SRR11074201_2.fastq.gz	Fastqsanger.gz
	SRR11074202_1.fastq.gz	Fastqsanger.gz

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RNA-seq of GAP43 overexpressed and control SW620 cancer cells

Project description:We found GAP43 was significantly downregulated in colorectal cancer. So we reintroduced GAP43 to colorectal cancer cell line, and investigated the influence to cancer cells. In order to get the transcriptome-wide impact of GAP43 overexpression in colorectal cancer, we made stable transfected GAP43 in SW620 cancer cells, and mRNA-seq were performed (Sequenced with PE150, and 20M reads were generated.) This study provides the DEGs of GAP43 overexpressed and control colorectal cancer cells.

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Project description:CD133 has been widely used for identification and isolation of cancer stem cells in tumors although its role as a marker for cancer stem cell is still controversial . We isolated the CD133+ and CD133- cells from SW620 human colon cancer cell line and compared their biological characteristics, such as tumorigenicity,drug sensitivity, etc. Our study revealed that CD133+ SW620 cells were more tumorigenic and resistant to anti-cancer drugs. Correspondingly, they displayed different gene expression profile. However, it was observed that CD133- cells and CD133+ cells could mutually convert, indicating that CD133 expression was under dynamic and reversible regulations which might impose significant infulence on cells behaviors. Thus, our data challenge the role of CD133 as a marker for cancer stem cell. There are two populations with distinct expression of CD133 in SW620 human colon cancer cell line. Microarray assays were employed to investigate the differentially expressed genes between the two populations, which may possess different tumorigenetic potential and sensitivity to anti-cancer drugs. CD133+ and CD133- cells were isolated from human colon cancer SW620 cell line by magnetic cell sorting system. The clones from sorted CD133+ or CD133- populations were established. Clone cells were expanded and were further purified by using CD133 cell isolation kit before microarray assays.

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Project description:Two isogenic human colorectal cancer cell lines (primary SW480 cell line and its lymph node metastatic variant SW620 cell line),as an in vitro metastatic model. We have demonstrated that SW620 cell line possesses high metastasis potential and SW480 cell linepossesses low metastatic potential. We want to compare the whole cell microRNAs profiles of two isogenic colorectal cancer cell lines (SW480 and SW620 cell line), to gain an insight into the molecular events of colon cancer metastasis.

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mRNA expression after knockdowwn of UICLM in SW620 cells

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2024-11-01 | GSE188329 | GEO

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