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MRNA expression profile of control and senescent human colorectal cancer cell line SW620

ABSTRACT: mRNA expression profile of control and senescent human colorectal cancer cell line SW620

PROVIDER: PRJNA942628 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR23759677_1.fastq.gz	Fastqsanger.gz
	SRR23759677_2.fastq.gz	Fastqsanger.gz
	SRR23759678_1.fastq.gz	Fastqsanger.gz
	SRR23759678_2.fastq.gz	Fastqsanger.gz
	SRR23759679_1.fastq.gz	Fastqsanger.gz

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mRNA expression profile of control and senescent human colorectal cancer cell line SW620

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Project description:Two isogenic human colorectal cancer cell lines (primary SW480 cell line and its lymph node metastatic variant SW620 cell line),as an in vitro metastatic model. We have demonstrated that SW620 cell line possesses high metastasis potential and SW480 cell linepossesses low metastatic potential. We want to compare the whole cell microRNAs profiles of two isogenic colorectal cancer cell lines (SW480 and SW620 cell line), to gain an insight into the molecular events of colon cancer metastasis.

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mRNA expression after knockdowwn of UICLM in SW620 cells

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Genes regulated by Sox2 in colorectal cancer cell line

Project description:Knockdown of Sox2 in SW620 colorectal cancer cells decrease their growth rates in vitro and in vivo in xenograft models. We used microarrays to detail the global programme of gene expression in Sox2 Knockdown sw620 cells compared with mock knockdown sw620 cells Sox2 knockdown sw620 cells and and mock knockdown sw620 cells were cultured in RPMI 1640 cell culture media for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the genes regulated by Sox2 in colorectal cell lines and end (T4) of gastrulation.

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RNA-seq of GAP43 overexpressed and control SW620 cancer cells

Project description:We found GAP43 was significantly downregulated in colorectal cancer. So we reintroduced GAP43 to colorectal cancer cell line, and investigated the influence to cancer cells. In order to get the transcriptome-wide impact of GAP43 overexpression in colorectal cancer, we made stable transfected GAP43 in SW620 cancer cells, and mRNA-seq were performed (Sequenced with PE150, and 20M reads were generated.) This study provides the DEGs of GAP43 overexpressed and control colorectal cancer cells.

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Single cell gene expression profiling of the SW620 colorectal cell line

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