Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing LSK cells after Cxxc5 knockdown

ABSTRACT: RNA sequencing LSK cells after Cxxc5 knockdown

PROVIDER: PRJNA606512 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11079931.fastq.gz	Fastqsanger.gz
	SRR11079932.fastq.gz	Fastqsanger.gz
	SRR11079933.fastq.gz	Fastqsanger.gz
	SRR11079934.fastq.gz	Fastqsanger.gz
	SRR11079935.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

RNA sequencing LSK cells after Cxxc5 knockdown

Project description:To study the effect of knockdown of Cxxc5 on stem/progenitor cells, we used retrovirus to knockdown Cxxc5 expression in the LSK cells. We used this as a surrogate to study the function of Cxxc5 in mouse/stem progenitor cells.

2020-02-14 | GSE145246 | GEO

LSK cells after Cxxc5 knockdown

Project description:LSK cells after Cxxc5 knockdown

| PRJNA606510 | ENA

Single-cell RNA-sequencing of differentiation myeloid cells

Project description:During granulocyte-macrophage colony-stmulating factor (GM-CSF) driven ex vivo myeloid differentiation of mouse hematopoietic stem/progenitor cells defined as Lineage negative cKit positive Sca-1 postive (LSK) cells, LSK cells after Cxxc5 knockdown makes more granulocytic cells than monocytic cells. To study this effect of Cxxc5 knockdown during myeloid cell differentiation, we performed a single-cell RNA sequencing of differentiating myeloid cells and analyzed the transcriptome of these cells.

2020-02-14 | GSE145247 | GEO

Expression data of Wnt3a stimulated K562 cells after CXXC5 overexpression or knockdown

Project description:CXXC5 inhibits the canonical Wnt signaling pathway Impact of CXXC5 on Wnt stimulated gene expression in K562 cells

2015-03-20 | GSE67060 | GEO

LSK cells after Cxxc5 knockdown

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-02-14 | GSE145248 | GEO

Gene expression profiling of control cohesin knockdown LSK cells

Project description:We performed RNA-sequencing in LSK cells (Lin(neg)/c-Kit(+)/Sca-1(+)) from shRNA mice carrying an shRNA for Renilla, Smc1a or Stag2. RNA-sequencing control (Renilla) and cohesin (Smc1a and Stag2) knockdown cells.

2015-09-16 | E-GEOD-72369 | biostudies-arrayexpress

CXXC5 stabilizes DNA methylation patterns in mouse embryonic stem cells [RNA-seq]

Project description:Mammalian genomes encode a family of 12 proteins that contain a CXXC zinc finger domain. These domains are known to bind to unmethylated DNA sequences rich in CpG dinucleotides. Most members of this family are large multi-domain proteins that function in the control of DNA methylation and histone methylation patterns. CXXC5 is a smaller member of the family, along with its closest homologue CXXC4. These two proteins lack known catalytic domains. Here we have characterized CXXC5 in mouse embryonic stem (ES) cells. We show that CXXC5 is a nuclear protein that interacts with 5-methylcytosine oxidases (TET proteins). Removal of CXXC5 from ES cells leads to very few changes in gene expression. CXXC5 extensively colocalizes with TET1 and TET2 at CpG islands. CXXC5 inactivation leads to a substantial reduction of DNA methylation levels that affects all genomic compartments including genic and intergenic regions and CpG island shores. No hypermethylation of CpG islands was observed. We propose a model in which CXXC5 serves as an anchor for TET proteins at CpG islands. In the absence of CXXC5, the 5-methylcytosine oxidases become dislodged from CpG islands and are enabled to induce genome-scale DNA demethylation. Thus, CXXC5 serves as a stabilizer of DNA methylation patterns.

2024-06-04 | GSE268885 | GEO

CXXC5 stabilizes DNA methylation patterns in mouse embryonic stem cells [ChIP-Seq]

2024-06-04 | GSE268884 | GEO

CXXC5 stabilizes DNA methylation patterns in mouse embryonic stem cells [Bisulfite-Seq]

2024-06-04 | GSE268886 | GEO

Gene expression profiling of control cohesin knockdown LSK cells

Project description:We performed RNA-sequencing in LSK cells (Lin(neg)/c-Kit(+)/Sca-1(+)) from shRNA mice carrying an shRNA for Renilla, Smc1a or Stag2.

2015-09-16 | GSE72369 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data