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Single-cell RNA-sequencing of differentiation myeloid cells

ABSTRACT: During granulocyte-macrophage colony-stmulating factor (GM-CSF) driven ex vivo myeloid differentiation of mouse hematopoietic stem/progenitor cells defined as Lineage negative cKit positive Sca-1 postive (LSK) cells, LSK cells after Cxxc5 knockdown makes more granulocytic cells than monocytic cells. To study this effect of Cxxc5 knockdown during myeloid cell differentiation, we performed a single-cell RNA sequencing of differentiating myeloid cells and analyzed the transcriptome of these cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE145247 | GEO | 2020/02/14

REPOSITORIES: GEO

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