Expression profiling of garlic genes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA607255 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR11100900_1.fastq.gz | Fastqsanger.gz | |||
| SRR11100900_2.fastq.gz | Fastqsanger.gz | |||
| SRR11100901_1.fastq.gz | Fastqsanger.gz | |||
| SRR11100901_2.fastq.gz | Fastqsanger.gz | |||
| SRR11100902_1.fastq.gz | Fastqsanger.gz |
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Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed C57BL/6J mice serendipitously infected with Pasteurella multocida and identified acute phase response genes, particularly Lcn2 and Orm2, as the major players in the innate response. Also dieraty garlic suppressed pasteurella infection in C57BL/6J mice.
2019-12-31 | GSE20093 | GEO
Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in control (cellulose) and garlic-fed mice and identified erythropoietic and heme biosynthetic genes that could, in part, be responsible for the pleiotropic effects of garlic on health Experiment Overall Design: 8-week old male C57BL/6J mice were divided into two diet groups and maintained at an SPF facility. Mice were fed (ad libitum) a special diet (Yeh and Yeh, 1994) with 4% cellulose (Control group, 3 mice) or 4% lyophilized raw garlic powder (Treatment group, 3 mice). At the end of the 15-week treatment, spleen organs were used for RNA isolation and arraying.
2009-01-15 | E-GEOD-10344 | biostudies-arrayexpress
Project description:Garlic is a popular flavor enhancer in modern cuisines. Although anti-atherosclerotic, anti-proliferative, hypolipidemic and chemopreventative effects of garlic are known for a long time, the mechanisms of garlic as a dietary supplement ramain largely unknown. We used microarrays to analyze the global programme of gene expression in the liver tissues of control (cellulose) and garlic-fed C57BL/6J mice maintained at an SPF (Specific Pathogen Free) facility.
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Project description:Alcohol induced fatty liver cause a dangerous health problem and is the major cause of morbidity and mortality worldwide. Garlic (Allium sativum) is documented to possess anti-fatty liver properties. However the exact molecular mechanisms are unknown. The main aim of this experiment is to elucidate the underlying pathways through which garlic ameliorates alcohol induced fatty liver. Dially disulfide and garlic oil were the garlic compounds used in this study. Leiber DeCarli ethanol liquid diet was to induce fatty liver in C57BL/6 mice model. Also the expression impaired by alcohol induced fatty liver is another aim of this study.
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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare transcriptome profiling of control of C. albicans ATCC 10231 (RNA-seq) to transcriptome profiling of garlic oil-treated C. albicans ATCC 10231 and to evaluate protocols for optimal high-throughput data analysis Methods:SDB medium, C. albicans cells and garlic oil were added to two separate 50 mL conical flasks. The initial cell concentrations in culture were both 105 CFU/mL. The cultures were incubated in a water bath shaker at 28 ºC with shaking at 150 rpm for 12 h. Garlic oil or PBS buffer were then added to the cultures to make the concentrations of garlic oil arrived at 0 (control) and 1.25 μl/mL, respectively. The cultures were continuously incubated at the same conditions for 5 h. The cells were then sampled and centrifuged. The cell precipitates in the control and 1.25 μl/mL garlic oil groups were quickly separately frozen at -80 ºC. Then total RNA were repared from the cell pellets. Results:The RNA sequencing results showed that many genes in C. albicans exposed to garlic oil were differentially expressed. Nearly three thousand genes were differentially expressed, with either an increase or decrease of more than twofold. Most of them were down regulated while a small number were upregulated. Conclusions: Our study indicated that garlic oil induced differential expression of some critical genes, such as genes for cellular response to drugs, oxidation-reduction processes, pathogenesis, and the cellular response to starvation. Moreover, the differentially expressed genes were mainly clustered in 19 KEGG pathways, such as oxidative phosphorylation, spliceosome, cell cycle, protein processing in endoplasmic reticulum, pyrimidine metabolism, meiosis, RNA transport, ribosome biogenesis, and RNA degradation.
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