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RNAseq analysis on e9.5 mutant mouse hearts

ABSTRACT: RNAseq analysis on e9.5 mutant mouse hearts

PROVIDER: PRJNA607311 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11103352_1.fastq.gz	Fastqsanger.gz
	SRR11103352_2.fastq.gz	Fastqsanger.gz
	SRR11103353_1.fastq.gz	Fastqsanger.gz
	SRR11103353_2.fastq.gz	Fastqsanger.gz
	SRR11103354_1.fastq.gz	Fastqsanger.gz

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RNAseq analysis on e9.5 mutant mouse hearts

Project description:We report RNAseq analysis on RNA from e9.5 hearts of Asb2 mutant and Asb2.Flna double mutants with controls. The goal of this study is to identify Asb2 downstream targets in the heart as well as targets that are corrected in the Asb2.Flna double mutant vs the Asb2 singe mutants.

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Project description:Purpose: The goal of this study is to identify the differential cardiac transcriptome profiling between WT and Smyd1 null (Smyd1-KO) hearts at E9.5 using RNA-seq. Methods: mRNA profiles of E9.5 WT and Smyd1-KO mouse hearts were generated by deep sequencing, n=3 for each genotype, using Illumina HiSeq2500. The sequence reads were aligned to the mm10 reference genome using STAR via the bcbio-nextgen RNA-sequencing pipeline. Differential gene expression was determined by DEseq2. Results: 1756 genes were differentially expressed between WT and Smyd1-KO hearts [adjusted P value <0.05, |log2(Fold Change)| > 0.5], with 1130 upregulated and 626 downregulated in E9.5 Smyd1-KO hearts.

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Project description:We have analysed and compared mRNA expression between wt embryos and embryos deficient for Arid3b in E9.5 hearts, with the aim of identifying differentially expressed genes that could give us a clue to the functions of Arid3b during development. Hearts were dissected from E9.5 embryos (including the heart tube and the pharyngeal mesoderm and endoderm located beneath). Four wild-type and four mutant embryos were collected and pooled for each of the microarray triplicates.

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