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Gene expression analysis of cultured bone marrow-derived eosinophils in response to GM-CSF and IL-10 stimulation

ABSTRACT: Gene expression analysis of cultured bone marrow-derived eosinophils in response to GM-CSF and IL-10 stimulation

PROVIDER: PRJNA608914 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11186551.fastq.gz	Fastqsanger.gz
	SRR11186552.fastq.gz	Fastqsanger.gz
	SRR11186553.fastq.gz	Fastqsanger.gz
	SRR11186554.fastq.gz	Fastqsanger.gz
	SRR11186555.fastq.gz	Fastqsanger.gz

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Gene expression analysis of cultured bone marrow-derived eosinophils in response to GM-CSF and IL-10 stimulation

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Project description:Background: Despite recent advances in asthma management with anti-IL-5 therapies, many patients with eosinophilic asthma remain poorly controlled. IL-3 shares a common beta subunit receptor with both IL-5 and GM-CSF, but through alpha subunit-specific properties, uniquely influences eosinophil biology and may serve as a potential therapeutic target. Objective: We aimed to globally characterize the transcriptomic profiles of GM-CSF, IL-3, and IL-5 stimulation and identify differences in gene expression using advanced statistical modeling. Methods: Human eosinophils were isolated from the peripheral blood of healthy volunteers and stimulated with either GM-CSF, IL-3 or IL-5 for 48 hours. RNA was then extracted and bulk sequencing performed. DESeq analysis identified differentially expressed genes and weighted gene co-expression network analysis independently defined modules of genes that are highly co-expressed. Results: IL-3 stimulation yielded the most numbers of differentially expressed genes that were also highly co-expressed. GM-CSF and IL-5 stimulation demonstrated redundancy in eosinophil gene expression. Conclusion: IL-3 produces a distinct eosinophil gene expression program among the beta-chain receptor cytokines.

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