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Single-cell transcriptomics during different phases post cardiac ischemia reveals secreted B2M to regulate fibroblast activation

ABSTRACT: Single-cell transcriptomics during different phases post cardiac ischemia reveals secreted B2M to regulate fibroblast activation

PROVIDER: PRJNA609983 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11219602_1.fastq.gz	Fastqsanger.gz
	SRR11219602_2.fastq.gz	Fastqsanger.gz
	SRR11219603_1.fastq.gz	Fastqsanger.gz
	SRR11219603_2.fastq.gz	Fastqsanger.gz
	SRR11219604_1.fastq.gz	Fastqsanger.gz

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Single-cell transcriptomics during different phases post cardiac ischemia reveals secreted B2M to regulate fibroblast activation

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Project description:<p>Renal fibrosis, a hallmark of chronic kidney diseases, is driven by the activation of renal fibroblasts. Recent studies have highlighted the role of glycolysis in this process. Nevertheless, one critical glycolytic activator, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), remains unexplored in renal fibrosis. Upon reanalyzing the single-cell sequencing data from Dr. Humphreys' lab, we noticed an upregulation of glycolysis, gluconeogenesis, and TGFβ signaling pathway in myofibroblasts from fibrotic kidneys after unilateral ureter obstruction (UUO) or kidney ischemia/reperfusion. Furthermore, our experiments showed significant induction of PFKFB3 in mouse kidneys following UUO or kidney ischemia/reperfusion. To delve deeper into the role of PFKFB3, we generated mice with Pfkfb3 deficiency specifically in myofibroblasts (Pfkfb3f/fPostnMCM). Following UUO or kidney ischemia/reperfusion, a substantial decrease of fibrosis in injured kidneys of Pfkfb3f/fPostnMCM mice was identified compared to their wild-type littermates. Additionally, in cultured renal fibroblast NRK-49F cells, PFKFB3 was elevated upon exposure to TGFβ1, accompanied by the increase of α-SMA and fibronectin. Notably, this upregulation was significantly diminished with PFKFB3 knockdown, correlated with a glycolysis suppression. Mechanistically, the glycolytic metabolite lactate promoted the fibrotic activation of NRK-49F. In conclusion, our study demonstrates the critical role of PFKFB3 in driving fibroblast activation and subsequent renal fibrosis.</p>

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