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Allyl isothiocyanate (AITC) trigged morphological, sensing process and transcription profiling alternation in Fusarium solani

ABSTRACT: Allyl isothiocyanate (AITC) trigged morphological, sensing process and transcription profiling alternation in Fusarium solani

PROVIDER: PRJNA611716 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11280166_1.fastq.gz	Fastqsanger.gz
	SRR11280166_2.fastq.gz	Fastqsanger.gz
	SRR11280167_1.fastq.gz	Fastqsanger.gz
	SRR11280167_2.fastq.gz	Fastqsanger.gz
	SRR11280168_1.fastq.gz	Fastqsanger.gz

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Allyl isothiocyanate (AITC) trigged morphological, sensing process and transcription profiling alternation in Fusarium solani

Project description:Allyl isothiocyanate (AITC) is a natural product used as a food additive. Due to its strong volatility and broad biological activity, AITC is being considered as a bio-fumigant to control soil-borne fungal diseases in agriculture, creating an urgent need for evaluation of the antifungal activity of AITC. Here, AITC-trigged growth inhibition, sensing, and related molecular mechanisms were analyzed in Fusarium solani by employing morphological, genetic, and transcriptional profiling analysis. The results indicated that AITC causes rapid inhibition of F. solani after 5 min, hyphal deformity, and electrolyte leakage. A yeast-like vacuolar transient receptor potential channel regulator (FsYvc1, a STRPC family member) was identified in F. solani that is play a switch role for sensing AITC. Genetic evidence suggests the gene FsYvc1 involved in growth, development, and pathogenicity. Loss of FsYvc1 resulted in hypersensitivity of F. solani to AITC and high levels of reactive oxygen species with weak responses to CaCl2, NaCl, KCl, SDS, and Congo red as well as triadimefon, triadimenol, chlorothalonil, and azoxystrobin. Transcription profiling and qRT-PCR showed that three transcripts were up-regulated in the wild type (WT) but down-regulated in the FsYvc1 mutants (∆FsYvc1), which were involved in TRP ion channel expression, biosynthetic process, and oxidoreductase activity. Three transcripts down-regulated in WT but up-regulated in the ∆FsYvc1 were involved in metabolic process, proper enzyme folding (GrpB protein), and the response to abiotic stress (WD40 repeat). This study provides novel insights into the toxicity mechanisms of AITC. Considering the key role of FsYvc1, it could act as a new molecular target for future fungicide development.

2020-03-11 | GSE146707 | GEO

Transcriptomic response of Sclerotinia sclerotiorum after exposure to allyl-isothiocyanate and indol-3 carbinol

Project description:We performed a transcriptomic analysis of the necrorophic fungus Sclerotinia sclerotiorum exposed to two different isothiocyanates (allyl-isothiocyanate and indol-3-carbinol), searching for mechanisms of adaptation and detoxification of these chemicals.

2020-12-29 | GSE144344 | GEO

Transcriptomic response of Xanthomonas campestris pv. campestris after exposure to allyl-isothiocyanate and indol-3 carbinol

Project description:We performed a transcriptomic analysis of the necrotrophic bacteria Xanthomonas campestris pv. campestris exposed to two different isothiocyanates (allyl-isothiocyanate and indol-3-carbinol), searching for mechanisms of adaptation and detoxification of these chemicals.

2021-09-08 | GSE167555 | GEO

Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defense responses in Arabidopsis thaliana

Project description:Isothiocyanates (ITCs) are degradation products of the plant secondary metabolites glucosinolates (GSLs) and are known to affect human health as well as plant herbivores and pathogens. To investigate the processes engaged in plants upon exposure to isothiocyanate we performed a genome scale transcriptional profiling of Arabidopsis thaliana at different time points in response to an exogenous treatment with allyl-isothiocyanate.

2016-10-27 | GSE81634 | GEO

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

Project description:We investigated antibacterial activities of benzyl isothiocyanate (BITC) against V. parahaemolyticus by transcriptomic analysis and morphological verification. Treatment with 1/8MIC BITC resulted in 234 up-regulated genes and 273 down-regulated genes. In V. parahaemolyticus, we selected six genes that were significantly down-regulated from the transcriptome results and verified them using quantitative Real-time Polymerase Chain Reaction (qRT-PCR). The verification experiments indicated that the relative expressions of the six virulence genes VP0820, VP0548, VP2233, VPA2362, fliA and fliG were only 31.0%, 31.1%, 55.8%, 57.0%, 75.3%, and 79.9% of the control group, respectively. Among them, VP2233, fliA and fliG are related to flagella, and VP2362 can regulate a protein related to biofilm formation. From the morphological aspect, we verified that under the action of 1/8 MIC BITC, the swimming diffusion diameter of V. parahaemolyticus was significantly reduced by 14.9%, and biofilm formation was significantly inhibited. These results indicate that 1/8 MIC BITC can reduce the pathogenicity of V. parahaemolyticus by inhibiting virulence gene expression related to flagella and biofilm, which is helpful for further understanding of bacteriostatic mechanism of BITC on V. parahaemolyticus and other food-borne pathogens.

2020-06-18 | GSE152671 | GEO

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

Project description:Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

| PRJNA640045 | ENA

Rhizoctonia solani AG8 infection of wheat seedling roots

Project description:Rhizoctonia solani is a nectrotrophic fungal pathogen that causes billions of dollars of damage to agriculture worldwide and infects a broad host range including wheat, rice, potato and legumes. In this study we identify wheat genes that are differentially expressed in response to the R. solani isolate, AG8-1, using microarray technology. A significant number of wheat genes identified in this screen were involved in ROS production and redox regulation. Levels of ROS species were increased in wheat root tissue following R. solani infection as determined by NBT, DAB and titanium sulphate measurements/stainings. Pathogen/ROS related genes from R. solani were also tested for expression patterns upon wheat infection. TmpL, a R. solani gene homologous to a gene associated with ROS regulation in Alternaria brassicicola, and OAH, a R. solani gene homologous to oxaloacetate acetylhydrolase which has been shown to produce oxalic acid in Sclerotinia sclerotiorum, were highly induced in R.solani when infecting wheat. We speculate that the wheat germin-like protein (GLP) is induced to inactivate the oxalic acid that is produced by the R. solani OAH.

2015-11-04 | GSE74671 | GEO

Rhizoctonia solani AG8 infection of wheat seedling roots

2015-11-04 | E-GEOD-74671 | biostudies-arrayexpress

Transcriptional characterization of bacteria-fungi interaction between Priestia endophytica FH5 and phytopathogen Rhizoctonia solani

Project description:Priestia endophytica FH5, which was isolated from healthy tomato rhizosphere soil, had biological activity against a variety of plant diseases, including R. solani. We isolated the chemicals generated by strain FH5 to better understand the interaction between strain FH5 and R. solani. A transcriptome study of strain FH5 with and without R. solani exposure was also performed. In response to the fungal pathogen R. solani, strain FH5 changed genes linked to amino acid transport, carbohydrate transport, energy generation and conversion, and inorganic ion transport and metabolism, according to our findings.

2021-07-09 | GSE179605 | GEO

Differentially expressed genes during the potato-Rhizoctonia solani AG3 interaction

Project description:Differential analysis of the potato-Rhizoctonia solani AG3 interaction. Samples were extracted from R. solani inoculated potato sprouts at two time points. R. solani is one of the most prominent fungal pests of potato and therefore of great economic relevance.

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