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Dnmt1 deletion in Hmx3-positive POA-derived interneurons

ABSTRACT: Dnmt1 deletion in Hmx3-positive POA-derived interneurons

PROVIDER: PRJNA612519 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR11305718.fastq.gz	Fastqsanger.gz
	SRR11305719.fastq.gz	Fastqsanger.gz
	SRR11305720.fastq.gz	Fastqsanger.gz
	SRR11305721.fastq.gz	Fastqsanger.gz
	SRR11305722.fastq.gz	Fastqsanger.gz

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Dnmt1 deletion in Hmx3-positive POA-derived interneurons [MeDIP-seq]

Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. MeDIP data are assigned here.

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Dnmt1 deletion in Hmx3-positive POA-derived interneurons

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Project description:The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed RNA-Seq and MeDIP-Seq.

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