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Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production.

ABSTRACT: Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production.

PROVIDER: PRJNA613488 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11353921.fastq.gz	Fastqsanger.gz
	SRR11353922.fastq.gz	Fastqsanger.gz
	SRR11353923.fastq.gz	Fastqsanger.gz
	SRR11353924.fastq.gz	Fastqsanger.gz
	SRR11353925.fastq.gz	Fastqsanger.gz

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Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production.

Project description:Acute liver failure is a clinical syndrome of severe hepatic dysfunction. Immune cells play an important role in the failure. In recent years, the immunoregulatory function of extracellular vesicles (EVs) has been reported; therefore, it is inferred that EVs have some role in an immune mediated liver injury. In this study, we investigated the immunoregulatory function of EVs in a concanavalin A (Con A)-induced liver injury. The mouse model was prepared by a single intravenous administration of 15 mg/kg Con A, in which there was a significant increase in serum EVs number. In in vitro study, the secreted EVs number was also significantly increased in Con A-treated RAW264.7, a mouse macrophage cell line, but not Hepa1-6, a mouse hepatoma cell line. In in vitro EVs treatment study, the EVs from Con A-treated mice serum and Con A-treated RAW264.7 suppressed the inflammatory cytokines production in Con A-stimulated RAW264.7. miRNA sequencing analysis showed that mmu-miR-122-5p and mmu-miR-148a-3p were commonly increased in these EVs and the EVs-treated cells. The enriched pathways in the miRNAs predicted target genes included the inflammatory response pathways. mRNA levels of the target genes in the pathways (mitogen-activated protein kinase, Phosphoinositide 3-kinase/Akt and Rho/Rho associated coiled-coil containing protein kinase pathways) were decreased in the EVs-treated cells. In in vivo RNA interference study, knock down of liver RAB27A, an EVs secretion regulator, significantly exacerbated the Con A-induced liver injury. These data suggest that macrophage-derived EVs play an important role in a Con A-induced liver injury through the immunoregulation.

2020-03-20 | GSE147243 | GEO

Effect of YTHDF1 on concanavalin A-induced hepatitis

Project description:To investigate the effect of YTHDF1 on concanavalin A (ConA)-induced hepatitis , we performed RNA-seq with the total RNA extracted from the moue liver. Ythdf1-/- or wild type mice were intravenously injected with ConA (8 mg/kg) or saline for 8 hours. Then, the livers were collected, homogenized, and subjected to total RNA extraction.

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Effect of a DP1 agonist BW245C on concanavalin A-induced hepatitis

Project description:To elucidate the effect of a prostaglandin D2 receptor DP1 agonist BW245C on concanavalin A (ConA) hepatitis, we performed DNA microarray using SurePrint G3 Mouse GE 8x60K Microarray. Mice were treated with ConA for 3 or 24 hr with vehicle or BW245C . Livers were collected, homogenized, and subjected to total RNA extraction.

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Effect of YTHDF1 on concanavalin A-induced hepatitis

Project description:Effect of YTHDF1 on concanavalin A-induced hepatitis

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stem cell therapy of murine acute hepatitis

Project description:Gene expression profiles of the liver and hepatic inflammatory cells in murine concanavalin A-induced acute hepatitis models by treatment with adipose tissue derived stem cells C57Bl/6 female mice were treated with adipose tissue-derived stem cells immediately or 3 hours after concanavalin A injection. Two hours later, the liver tissues or hepatic inflammatory cells were obtained and gene expression of them were examined by DNA microarray.

2012-10-11 | E-GEOD-41465 | biostudies-arrayexpress

stem cell therapy of murine acute hepatitis

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MicroRNA-674-5p/5-LO axis is important in concanavalin A-induced acute mouse liver injury

Project description:Eight-week-old male pathogen-free BALB/c mice were used for the induction of concanavalin A (Con A)-induced hepatitis. Animals were acclimated for at least 6-7 days to a 12-hour light/dark cycle in a humidity- and temperature-controlled, specific-pathogen-free environment in autoclaved cages. Mice were randomly assigned to two groups (4 in each group) and received Con A type IV (or saline) intravenously via the tail vein at the dose of 20 mg/kg for 2 hours. The animals were sacrificed for blood and liver collection. Blood was taken for transaminase and cytokine measurements. Livers were excised immediately after sacrifice. Part of the livers were immediately snap-frozen in liquid nitrogen and kept at -80Â°C for total RNA isolation and microRNA microarray analysis. miR-674-5p and other miRNAs were experimentally validated to be dysregulated by Con A in mouse livers. Liver samples from four mice treated with Con A and four mice treated with saline were used to acquire the miRNA expression profiling.

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Project description:RNA sequencing of mouse liver tissues 24 hours after Concanavalin A (Con A) administration.

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Project description:Eight-week-old male pathogen-free BALB/c mice were used for the induction of concanavalin A (Con A)-induced hepatitis. Animals were acclimated for at least 6-7 days to a 12-hour light/dark cycle in a humidity- and temperature-controlled, specific-pathogen-free environment in autoclaved cages. Mice were randomly assigned to two groups (4 in each group) and received Con A type IV (or saline) intravenously via the tail vein at the dose of 20 mg/kg for 2 hours. The animals were sacrificed for blood and liver collection. Blood was taken for transaminase and cytokine measurements. Livers were excised immediately after sacrifice. Part of the livers were immediately snap-frozen in liquid nitrogen and kept at -80°C for total RNA isolation and microRNA microarray analysis. miR-674-5p and other miRNAs were experimentally validated to be dysregulated by Con A in mouse livers.

2016-06-27 | GSE76345 | GEO

Role of Hif1alpha in cytokine-induced macrophage gene expression

Project description:Role of Hif1alpha in cytokine-induced macrophage gene expression

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