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Effect of YTHDF1 on concanavalin A-induced hepatitis

ABSTRACT: Effect of YTHDF1 on concanavalin A-induced hepatitis

PROVIDER: PRJNA998401 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25420582.fastq.gz	Fastqsanger.gz
	SRR25420583.fastq.gz	Fastqsanger.gz
	SRR25420584.fastq.gz	Fastqsanger.gz
	SRR25420585.fastq.gz	Fastqsanger.gz
	SRR25420586.fastq.gz	Fastqsanger.gz

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Effect of YTHDF1 on concanavalin A-induced hepatitis

Project description:To investigate the effect of YTHDF1 on concanavalin A (ConA)-induced hepatitis , we performed RNA-seq with the total RNA extracted from the moue liver. Ythdf1-/- or wild type mice were intravenously injected with ConA (8 mg/kg) or saline for 8 hours. Then, the livers were collected, homogenized, and subjected to total RNA extraction.

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Project description:10plex TMT labelling quantification expriments were performed to access the knockdown effect of YTHDF1 in cells.3 shGFPs control samples, 3 YTHDF1 shRNA-2 knockdown samples , 3 YTHDF1 shRNA-3 knockdown samples and 1 mixture sample were labelled by TMT 10-plex reagents.

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Project description:Eight-week-old male pathogen-free BALB/c mice were used for the induction of concanavalin A (Con A)-induced hepatitis. Animals were acclimated for at least 6-7 days to a 12-hour light/dark cycle in a humidity- and temperature-controlled, specific-pathogen-free environment in autoclaved cages. Mice were randomly assigned to two groups (4 in each group) and received Con A type IV (or saline) intravenously via the tail vein at the dose of 20 mg/kg for 2 hours. The animals were sacrificed for blood and liver collection. Blood was taken for transaminase and cytokine measurements. Livers were excised immediately after sacrifice. Part of the livers were immediately snap-frozen in liquid nitrogen and kept at -80Â°C for total RNA isolation and microRNA microarray analysis. miR-674-5p and other miRNAs were experimentally validated to be dysregulated by Con A in mouse livers. Liver samples from four mice treated with Con A and four mice treated with saline were used to acquire the miRNA expression profiling.

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