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Expression data from Neuro2a cells treated with siRNA targeting to Cpeb1 mRNA.

ABSTRACT: Expression data from Neuro2a cells treated with siRNA targeting to Cpeb1 mRNA.

PROVIDER: PRJNA615678 | ENA |

REPOSITORIES: ENA

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Expression data from Neuro2a cells treated with siRNA targeting to Cpeb1 mRNA.

Project description:Cytoplasmic polyadenylation element binding protein 1 (CPEB1) regulates polyadenylation and subsequent translation of CPE-containing mRNAs, which contributes to various physiological and pathological phenomena. To clarify changes in gene expression profiles caused by dysregulation of CPEB1 expression, we performed microarray analyses and revealed that reduced CPEB1 expression altered the expression levels of numerous genes. These findings indicate that accurate post-transcriptional regulation of CPEB1 expression contributes to the maintenance of global gene expression partially through modulating lncRNA expression.

2021-03-31 | GSE147604 | GEO

siRNA profiling of mouse Neuro2a transfected with mKIAA1583 (Tmem132a) vs. control

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2008-06-14 | E-GEOD-5676 | biostudies-arrayexpress

Ribosome profiling analysis of Neuro2a cells with Ebp1 knockdown

Project description:We perform Ribosome Profiling (Riboseq) analysis of mouse Neuro2a neuronal cultures in Ebp1-siRNA knockdown vs. scrambled-siRNA control conditions in biological triplicate to assess the translation-specific function of Ebp1

2021-03-23 | GSE157423 | GEO

Cynomolgus macaques treated with Divalent-siRNA (Di-siRNA) targeting Huntingtin gene

Project description:Cynomolgus macaques treated with Divalent-siRNA (Di-siRNA) targeting Huntingtin gene

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Genome-wide mRNA targets of CPEB1 in muscle stem cells

Project description:We performed CPEB1 RIP-seq on freshly isolated muscle stem cells. We found that CPEB1 associated genes are enriched in translational regulation pathways.

2021-11-02 | GSE148912 | GEO

Prostate cancer cell lines: non-targeting siRNA versus SChLAP1-siRNA treated

Project description:SChLAP1 is a novel long non-coding RNA expressed in prostate cancer. Here we performed transcriptional profiling of the prostate cancer cell lines LNCaP and 22Rv1 comparing non-targeting siRNA treatment versus SChLAP1-siRNA treatment. Goal was to determine the effect of SChLAP1 knockdown on gene expression in prostate cancer. Two-condition experiment: non-targeting siRNA versus SChLAP1 siRNA treated cells. Biological replicates: 1 control replicate, 2 treatment replicates. Technical replicates: 3 replicates per SChLAP1 siRNA. Cell lines: 22Rv1 and LNCaP.

2013-07-20 | E-GEOD-40383 | biostudies-arrayexpress

mKIAA1583 (Tmem132a) siRNA interference

Project description:Gene expression profiling comparisons of Neuro2a transfected with either mKIAA1583 (Tmem132a) siRNA or control (non-specific) siRNA. Keywords: siRNA, gene expression

2008-04-09 | GSE5676 | GEO

Cynomolgus macaques treated with Divalent-siRNA (Di-siRNA) targeting Huntingtin gene

Project description:Examine potential off-target effects of HTT targeting siRNA in cynomologus macaque brains.

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Prostate cancer cell lines: non-targeting siRNA versus SChLAP1-siRNA treated

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CPEB1 or CPEB1 (T171A, S177A) interacting proteome analysis on C2C12 cells

Project description:CPEB1 regulates cellular function by post-transcriptionally controlling its targeted transcripts' translation. After bound to CPEs, CPEB1 recruits cytoplasmic poly (A) polymerase GLD2 to elongate the poly (A) tail for the maintenance of mRNA stability. The stability of mRNA is positively correlated with translational output. It is unexamined whether CPEB1 interacts with translation machinery to regulation translation. Previous reports demonstrate that phosphorylation is required for its regulation on translation. However, it is not well understood whether phosphorylation is essential for CPEB1 interaction with translation machinery or not. Here, we performed mass spectrometry on C2 cells transfected with CPEB1-mVenus or CPEB1 (T171A, S177A)-mVenus after IgG or mVenus antibody immunoprecipitation. After analysis, we identify CPEB1 interacting proteins and the phosphorylation dependent interacting proteins such as ribosomal proteins. Thus, our data suggest that CPEB1 regulate translation by interacting with translation machinery in a phosphorylation dependent manner.

2021-11-04 | PXD020822 | Pride

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