Lotmaria passim
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA62029 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| Other | ||||
| SRR27109702_1.fastq.gz | Fastqsanger.gz | |||
| SRR27109702_2.fastq.gz | Fastqsanger.gz | |||
| SRR27109703_subreads.fastq.gz | Fastqsanger.gz | |||
| SRR30920710_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 26 |
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Project description:This dataset contains nanoLC-MS/MS results of heads of honey bee workers. All the heads were from 13 days old bees that were 11 days postinfection (DPI) that lasted 24 hours. Abdomens of bees were analyzed using qPCR to determine parasite load. Further, corresponding selected heads were subjected to a label-free proteomic analysis. Overall, five heads of bees were selected from each of the following experimental exposures performed: i) control without L. passim and IMI exposure and with confirmed no L. passim detected; ii) bees positive for L. passim from the experimental exposure of L. passim; and iii) bees positive for L. passim from the experimental exposure of L. passim combined with 2.5 uL IMI coexposure. This research was supported by project no. QK1910018 (NAZV) and Institutional Support for R&D no. MZE-RO0423 of the Ministry of Agriculture of the Czech Republic.
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Project description:Two trypanosomatid species, Lotmaria passim and Crithidia mellificae, have been shown to parasitize honey bees to date. L. passim appears to be more prevalent than C. mellificae and specifically infects the honey bee hindgut. Although the genomic DNA has been sequenced, the effects of infection on honey bee health and colony are poorly understood. To identify the genes that are important for infecting honey bees and to understand their functions, we applied the CRISPR/Cas9 system to establish a method to manipulate L. passim genes. By electroporation of plasmid DNA and subsequent selection by drug, we first established an L. passim clone expressing tdTomato or Cas9. We also successfully disrupted the endogenous miltefosine transporter and tyrosine aminotransferase genes by replacement with drug (hygromycin) resistant gene using the CRISPR/Cas9-induced homology-directed repair pathway. The L. passim clone expressing fluorescent marker, as well as the simple method for editing specific genes, could become useful approaches to understand the underlying mechanisms of honey bee-trypanosomatid parasite interactions.
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Project description:Nosema ceranae and Lotmaria passim are two commonly encountered digestive tract parasites of the honey bee that have been associated with colony losses in Canada, the United States, and Europe. Though honey bees can be co-infected with these parasites, we still lack basic information regarding how they impact bee health at the individual and colony level. Using locally-isolated parasite strains, we investigated the effect of single and co-infections of these parasites on individual honey bee survival, and their responsiveness to sucrose. Results showed that a single N. ceranae infection is more virulent than both single L. passim infections and co-infections. Honey bees singly infected with N. ceranae reached < 50% survival eight days earlier than those inoculated with L. passim alone, and four days earlier than those inoculated with both parasites. Honey bees infected with either one, or both, parasites had increased responsiveness to sucrose compared to uninfected bees, which could correspond to higher levels of hunger and increased energetic stress. Together, these findings suggest that N. ceranae and L. passim pose threats to bee health, and that the beekeeping industry should monitor for both parasites in an effort correlate pathogen status with changes in colony-level productivity and survival.
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