Proteomics

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Imidacloprid increases the prevalence of Lotmaria passim in honey bee workers


ABSTRACT: This dataset contains nanoLC-MS/MS results of heads of honey bee workers. All the heads were from 13 days old bees that were 11 days postinfection (DPI) that lasted 24 hours. Abdomens of bees were analyzed using qPCR to determine parasite load. Further, corresponding selected heads were subjected to a label-free proteomic analysis. Overall, five heads of bees were selected from each of the following experimental exposures performed: i) control without L. passim and IMI exposure and with confirmed no L. passim detected; ii) bees positive for L. passim from the experimental exposure of L. passim; and iii) bees positive for L. passim from the experimental exposure of L. passim combined with 2.5 uL IMI coexposure. This research was supported by project no. QK1910018 (NAZV) and Institutional Support for R&D no. MZE-RO0423 of the Ministry of Agriculture of the Czech Republic.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Lotmaria Passim (ncbitaxon:1620387) Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Tomas Erban  

PROVIDER: MSV000092818 | MassIVE |

SECONDARY ACCESSION(S): PXD045086

REPOSITORIES: MassIVE

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Publications

Imidacloprid increases the prevalence of the intestinal parasite Lotmaria passim in honey bee workers.

Erban Tomas T   Parizkova Kamila K   Sopko Bruno B   Talacko Pavel P   Markovic Martin M   Jarosova Jana J   Votypka Jan J  

The Science of the total environment 20230911


A challenge in bee protection is to assess the risks of pesticide-pathogen interactions. Lotmaria passim, a ubiquitous unicellular parasite in honey bees, is considered harmful under specific conditions. Imidacloprid causes unpredictable side effects. Research indicates that both L. passim and imidacloprid may affect the physiology, behavior, immunity, microbiome and lifespan of honey bees. We designed cage experiments to test whether the infection of L. passim is affected by a sublethal dose of  ...[more]

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